Sensor of pyrophosphate and SNP typing sensor using the same

Details Sensor of pyrophosphate and SNP typing sensor using the same Sensor of pyrophosphate and SNP typing sensor using the same

2008-04-30

2009-12-15

Olsen, Kaj K (Department: 1795)

Electrolysis: processes, compositions used therein, and methods

Electrolytic analysis or testing

Involving enzyme or micro-organism

C204S403030, C204S403140, C435S006120

Reexamination Certificate

active

07632392

ABSTRACT:
A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate1; an electrode system that is formed thereon and has a measurement electrode2and a counter electrode3; and a plurality of reaction reagent layers that are provided on the substrate1and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer36including the enzyme being separated from reaction reagent layer35including the buffer component, and reaction reagent layer37including glyceraldehyde-3-phosphate being separated from the reaction reagent layer35including the buffer component.

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