Method for extracellular production of target proteins...

Details Method for extracellular production of target proteins... Method for extracellular production of target proteins...

2003-06-19

2009-02-17

Minnifield, N. M (Department: 1645)

Chemistry: molecular biology and microbiology

Vector, per se

C435S069100, C435S069700, C435S071100, C435S071200, C435S091400, C435S252300, C435S252330, C435S471000, C435S476000

Reexamination Certificate

active

07491528

ABSTRACT:
The present invention provides an expression vector comprising genes encoding OmpF ofE. coliand a desired protein,E.colitransformed with the expression vector, and a method for extracellular production of desired proteins by employing the same. The recombinant expression vector of the invention comprises an ampicillin-resistance gene, the OmpF promoter and the OmpF gene. In accordance with the invention, a desired protein can be produced extracellularly by a simpler method than conventional methods such that: secretory production of OmpF fusion protein begins simultaneously with growth of the cells through constitutive expression employing an OmpF promoter, and as the concentration of cells increases, the amount of secretory production of the protein also increases continuously. Therefore, desired proteins can be produced in large quantities by a high concentration culture of cells.

REFERENCES:
patent: 5658757 (1997-08-01), Haake et al.
patent: 5840518 (1998-11-01), Morishita et al.
patent: 6274345 (2001-08-01), Lee et al.
patent: 7070989 (2006-07-01), Lee et al.
patent: 7186525 (2007-03-01), Sakanyan et al.
patent: 2005/0019857 (2005-01-01), Lee et al.
patent: 2005/0207977 (2005-09-01), Reinl et al.
patent: 2006/0218667 (2006-09-01), Vojdani et al.
patent: WO 87/06953 (1987-11-01), None
patent: WO 94/25592 (1994-11-01), None
patent: WO 97/26359 (1997-07-01), None
patent: WO 03/016538 (2003-02-01), None
patent: WO 2005/123924 (2005-12-01), None
Park et al, Biotechnol. Prog., 1999, 15:164-167.
Makrides, Microbiological Reviews, Sep. 1996, 60/3:512-538.
Kitai et al, Appl. Microbiol. Biotechnol., 1988, 28:52-56.
Elfassi et al, PNAS, Apr. 1986, 83:2219-2222.
Kim et al, Biotechnology Letters, Aug. 1998, 20/8:799-803.
Kleman et al, Current Biology, 1994, 5:180-186.
Hellmuth et al, J. Biotechnology, 1994, 32:289-298.
Nagahari et al, EMBO Journal, 1985, 4/13A:3589-3592.
Lee, Trends in Biotechnol., Mar. 1996, 14:98-105.
Kern et al, Gene, 1995, 163:53-57.
Yoon et al, Biotechnology and Bioengineering, Mar. 30, 2003, 81/7:753-767.
Jeong et al, Applied and Environmental Microbiology, Jul. 1999, 65/7:3027-3032.
Jeong et al, Applied and Environmental Microbiology, Oct. 2002, 68/10:4979-4985.
Simula et al, Toxicology, Oct. 5, 1993, 82/1-3:3-20 abstract only.
Hahn et al, FEMS Immunology and Medical Microbiology, 1998, 20:111-119.
Weinstock et al, PNAS, USA, Jul. 1983, 80:4432-4436.
Lawrence et al, Infection and Immunity, Apr. 1990, 58/4:970-977.
Inokuchi et al, Nucleic Acid Research, 1982, 10/21:6957-6968.
Hoffman et al, PNAS, USA, Aug. 1985, 82:5107-5111.
Choi et al, Chemical Ingineering Science, 2006, 61:876-885.
Shibui, et al., High-level secretion of human Apolipoprotein E Produced inEscherichia coli: use of a secretion plasmid containing tandemly polymerized ompF-hybrid gene., 1991, Journal of Biotechnology, 17 109-120.
Shibui, et al., Secretion of a fuctional Fab fragment inEscherichia coliand the influence of culture conditions., 1992, Applied Microbiology Biotechnology, 37: 352-357.

Affiliated with

Also associated with

Rating

Method for extracellular production of target proteins... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for extracellular production of target proteins..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for extracellular production of target proteins... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4128305