Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-10-07
2009-10-13
Riley, Jezia (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C536S023100, C536S024300
Reexamination Certificate
active
07601503
ABSTRACT:
Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly(3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hybridized) oligonucleotides.
REFERENCES:
patent: 7083928 (2006-08-01), Leclerc et al.
patent: 1278 348 (1972-06-01), None
patent: WO 98/03499 (1998-01-01), None
patent: WO 99/26729 (1999-06-01), None
Asanov et al., “Effect of surface electrostatics on adsorption behavior and biospecific interactions of dna oligonucleotides,” SPIE, 5803: 170-177, 1999.
Balanda et al., “Watersoluble and blue luminescent cationic polyelectrolytes based on Ipoly (p-phenylene),” Macromolecules, 32:3970-3978, 1999.
Bauerle, et al., “Specific recognition of nucleobase-functionalized polythiophenes,” Adv. Mater., 3:324-330, 1998.
Bonhote et al., “Hydrophobic, highly conductive ambient-temperature molten salts,” lnorg. Chem., 35:1168-1178, 1996.
Bronich et al.., “Recognition of dna topology in reactions between plasmid dna and cationic copolymers,” J American Chem. Soc., 122:8339-8343, 2000.
Chayer et al., “Highly conducting water-soluble polythiophene derivatives,” Chem. Mater., 9:2902-2905, 1997.
Chen et al., “Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer,” PNAS, 96:122287-12292, 1999.
Chrisey et al., “Covalent attachment of synthetic dna to self-assembled monolayer films,” Nucleic Acids Research, 24:3031-3039, 1996.
Ewbank et al., “Amine functionalized polythiophenes:synthesis and formation of chiral, ordered structures on dna substrates,” Tetrahedron Letters, 42:155-157, 2001.
Faid, et al., “Responsive supramolecular polythiophene assemblies,” J. Am. Chem. Soc., 120:5274-5278, 1998.
Faid and Leclerc, “Functionalized regioregular polythiophenes: towards the development of biochromic sensors,” Chem. Comm., 2761-2762, 1996.
Flink at al., “Self-assembled monolayers on gold for applications in chemical sensing,” Reinhoudt in Sensors Update, Ed. H. Baltes, W. Gopel, J. Hesse, 8:3-19, 2001.
Fodor et al., “Light-directed, spatially addressable parallel chemical synthesis,” Science, 251:767-773, 1991.
Garnier et al “Toward intelligent polymers: dna sensors based on oligonucleotide-functionalized polypyrroles,” Synthetic Metals, 100:89-94, 1999.
International Search Report of International Application No. PCT/CA/02/00485 mailed Jan. 14, 2003.
Kassmi et al., “Aromatic nucleophilic substitution on thiophene rings,” J. of Molecular Catalysis, 72:299-305, 1992.
Korri-Youssoufi et al., “Toward bioelectronics: specific dna recognition based on an oligonucleotide-functionalized polypyrrole,” J. Am. Chem. Soc., 119:7388-7389, 1997.
Kumar at al., “Silanized nucleic acids: a general platform for dna immobilization,”Nucleic Acids Research, 28:e71, 2000.
Leclerc, “Optical and electrochemical transducers based on functionalized conjugated polymers,” Adv. Mater., 11:1491-1498; 1999.
Lenigk at al., “Surface characterization of a silicon-chip-based dna microarray,” Langmuir, 17:2497-2501, 2001.
Livache et al., “Preparation of a dna matric via an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing a pyrrole group,” Nucleic Acids Research, 22:2915-2921, 1994.
Lucas at al., “Expedient synthesis of symmetric aryl ketones and of ambient-temperature molten salts of imidazole,” Synthesis, 9:1253-1258, 2000.
Lukkari et al., “Polyelectrolyte multilayers prepared from water-soluble poly(alkoxythiophene) derivatives,” J. Am. Chem. Soc., 123:6083-6091, 2001.
Lvov at al., “Assembly of thin films by means of successive deposition of alternate layers of dna and poly(allylamine),” Macromolecules, 26:5396-5399, 1993.
Lvov at al., “Direct electrochemistry of myoglobin and cytochrome P450 cam in alternate layer-by-layer films with dna and other polyions,” J. Am. Chem. Soc., 120:4073-4080, 1998.
McQuade et al., “Conjugated polymer-based chemical sensors,” Chem. Rev., 100:2537-2574, 2000.
Mikkelsen, “Electrochemical biosensors for DNA sequence detection,” Electroanalysis, 8:15-19, 1996.
Peterlinz et al., “Observation of hyridization and dehybridization of thiol-tethered dna using two-color surface plasmon resonance spectroscopy,” J. Am. Chem. Soc., 119:3401-3402, 1997.
Peterson et al., “The effect of surface probe density on dna hybridization,” Nucleic Acids Research, 29:5163-5168, 2001.
Rogers et al., “Immobilization of oligonucleotides onto a glass support via disulfide bonds: a method for preparation of dna microarrays,” Analytical Biochemistry, 266:23-30, 1999.
Steel et al., Immobilization of nucleic acids at solid surfaces: effect of oligonucleotids length on layer assembly, Biophysical Journal, 79:975-981, 2000.
Taton et al., “Scanometric dna array detection with nanoparticle probes,” Science, 289:1757-1760, 2000.
Tyagi, et al., “Molecular beacons: probes that fluoresce upon hybridization,” Nature Biotechnology, 14:303-308, 1996.
Zammatteo et al., “Comparison between different strategies of covalent attachment of dna to glass surfaces to build dna microarrays,” Analytical Biochemistry, 280:143-150, 2000.
Boissinot Maurice
Ho Hoang Ahn
Leclerc Mario
GeneOhm Sciences Canada, Inc.
Knobbe Martens Olson & Bear LLP
Riley Jezia
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