Metabolic engineering of amino acid production

Details Metabolic engineering of amino acid production Metabolic engineering of amino acid production

2007-10-30

2009-12-22

Wang, Andrew (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C435S115000, C435S116000, C435S252300, C435S471000

Reexamination Certificate

active

07635579

ABSTRACT:
The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be quantified by the formula: [(g amino acid produced/g dextrose consumed)*100]=% Yield and expressed as yield from dextrose. The invention provides microorganisms that are made auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids by mutagenesis and selected for their ability to produce higher percent yields of the desired amino acid than the parental strain. Preferred microorganisms areCorynebacterium, BrevibacteriumorEscherichia coliproducing L-lysine. Mutagenesis is performed by classical techniques or through rDNA methodology. Methods of the invention are designed to increase the production of an amino acid by mutagenizing a parental strain, selecting cells auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids and selecting branched chain amino acid auxotrophs or bradytrophs that produce a higher percent yield from dextrose of the desired amino acid than the parental strain. Processes of the invention are designed for the production an amino acid comprising culturing in a medium a microorganism obtained by mutagenizing a parent strain to be auxotrophic or bradytrophic for branched chain amino acid synthesis and selecting variants that are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain.

REFERENCES:
patent: 2979439 (1961-04-01), Kinoshita et al.
patent: 3527672 (1970-09-01), Kubota et al.
patent: 3565951 (1971-02-01), Ishida et al.
patent: 3687810 (1972-08-01), Kurihara et al.
patent: 3700557 (1972-10-01), Nakayama et al.
patent: 3707441 (1972-12-01), Shiio et al.
patent: 3708395 (1973-01-01), Nakayama et al.
patent: 3825472 (1974-07-01), Kubota et al.
patent: 3970519 (1976-07-01), Tsuchida et al.
patent: 4169763 (1979-10-01), Nakayama et al.
patent: 4442208 (1984-04-01), Tsuchida et al.
patent: 4560654 (1985-12-01), Miwa et al.
patent: 4601983 (1986-07-01), Nakamori et al.
patent: 4656135 (1987-04-01), Tsuchida et al.
patent: 5118619 (1992-06-01), Scheer et al.
patent: 5188948 (1993-02-01), Katsurada et al.
patent: 5236831 (1993-08-01), Katsumata et al.
patent: 5521074 (1996-05-01), Katsumata et al.
patent: 5650304 (1997-07-01), Ishii et al.
patent: 5763231 (1998-06-01), Ono et al.
patent: 5766925 (1998-06-01), Sugimoto et al.
patent: 5846790 (1998-12-01), Kimura et al.
patent: 0 205 849 (1986-12-01), None
patent: 0 780 476 (1997-06-01), None
patent: 63 173592 (1988-07-01), None
Bröer, S. and Krämer, R., “Lysine excretion byCorynebacterium glutamicum. 2. Energetics and mechanism of the transport system,”Eur. J. Biochem. 202:137-143 Blackwell Sciences Ltd., Oxford, England (1991).
Bröer S. et al., “Strains ofCorynebacterium glutamicumwith Different Lysine Productivities May Have Different Lysine Excretion Systems,”Applied and Environmental Microbiology59:316-321 American Society for Microbiology, Washington, D.C. (1993).
Cordes, C. et al., “Cloning organization and functional analysis ofilvA, ilvB and ilvCgenes fromCorynebacterium glutamicum,” Gene112:113-116 Elsevier Science BV, Amsterdam, Netherlands (1992).
Eikmanns, B.J. et al., “Molecular aspects of lysine, threonine, and isoleucine biosynthesis inCorynebacterium glutamicum,” Antonie van Leeuwenhoek 64:145-163 Kluwer Academic Publishers, Netherlands (1993).
Keilhauer, C. et al., “Isoleucine Synthesis inCorynebacterium glutamicum: Molecular Analysis of theilvB-ilvN-ilvCOperon,”J. Bacteriology 175:5595-5603 American Society for Microbiology, Washington, D.C. (1993).
Kinoshita,, S. et al., “Glutamic Acid Fermentation,” inProceedings of the International Symposium on Enzyme Chemistry, Pan-Pacific Press, Tokyo, Japan, pp. 464-468 (1958).
Kleemann, A. et al., “Amino Acids” inUllmann's Encyclopedia of Industrial Chemistry. vol. A2, Gerhartz, W. et al., eds., VCH-Verlagsgesellschaft, Weinheim, Germany, pp. 57-97 (1985).
Möckel, B. et al., “Functional and Structural Analyses of Threonine Dehydratase fromCorynebacterium glutamicum,” J. Bacteriology 174:8065-8072 American Society for Microbiology, Washington, D.C. (1992).
Nakayama, K. et al., “Microbial Production of Essential Amino Acids withCorynebacterium glutamicummutants,” inNutritional Improvement of Food and Feed Proteins,Friedman, M., ed., Plenum Press, New York, NY, pp. 649-661 (1978).
Sahm, H. et al., “Construction of L-Lysine-, L-Threonine-, or L-Isoleucine-Overproducing Strains ofCorynebacterium glutamicum,” Ann. N.Y. Acad. Sci 782:25-39 New York Academy of Sciences, New York, NY (1996).
Van Walsem, H.J. & Thompson, M.C., “Simulated moving bed in the production of lysine,”J. Biotechnol. 59:127-132 Elsevier Science BV, Amsterdam, Netherlands (1997).
International Search Report for International Application No. PCT/US00/20979, mailed Oct. 25, 2000.
Dialog File 351, Accession No. 7605632, Derwent WPI English language abstract for JP 63 173592.
Dialog File 351, Accession No. 11344245, Derwent WPI English language abstract for EP 0 780 476 A.

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