Spectrophotometric analysis of plasma in a closed-container

Details Spectrophotometric analysis of plasma in a closed-container Spectrophotometric analysis of plasma in a closed-container

2005-02-24

2009-12-29

Wallenhorst, Maureen M (Department: 1797)

Chemistry: analytical and immunological testing

Including sample preparation

Liberation or purification of sample or separation of...

C436S045000, C436S063000, C436S164000, C436S165000, C436S171000, C436S174000

Reexamination Certificate

active

07638342

ABSTRACT:
The present invention provides a method of analyzing plasma from a sample of whole blood. The method includes a) obtaining a sample of whole blood from a subject in a container, the container having a first end and a second end, the first end of the container for receiving a sample of whole blood; b) centrifuging the container under conditions to separate the whole blood into a cell layer, a gel layer and a plasma layer, wherein the centrifuging produces in relative juxtaposition the first end of the container, the cell layer, the gel layer, the plasma layer and the second end of the container; and c) inserting into a spectrophotometric device the second end of the container which includes the plasma layer in closest relative juxtaposition, to analyze the plasma from the whole blood.

REFERENCES:
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patent: 3951801 (1976-04-01), Ayres
patent: 4119407 (1978-10-01), Goldstein et al.
patent: 6706536 (2004-03-01), Carroll et al.
patent: 0 001 200 (1979-03-01), None
Copeland, B.E., M.D., et al., “Hemoglobin by First Derivative Spectrophotometry: Extent of Hemolysis . . . ,” Annals of Clinical and Laboratory Science 9:383-388(1969).

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