Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1985-10-21
1987-10-13
Scott, Samuel
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
435294, 435 30, 435300, 73863, 222420, 422100, C12M 100
Patent
active
046998845
DESCRIPTION:
BRIEF SUMMARY
The invention relates to a process for the simultaneous application of a multiplicity of liquid samples to an object stage, more especially of sera on an object stage overgrown with cell cultures, as well as to an apparatus for carrying out the process.
For the purposes of the microscopic investigation of antibodies, attached to antigens of a cell culture, from an applied serum, it is known to provide an object stage of the standard size 26.times.76 mm or larger with applied cell cultures and to inoculate the latter with serum with the aid of a pipette. For this purpose, in many cases multipipettes are used, which permit the simultaneous application of a multiplicity of serum samples. With the aid of these multipipettes, the cell cultures themselves can also be applied to the object stage in advance.
It requires a high degree of concentration and skill to apply the cell cultures in a relatively close pattern to an object stage and then to inoculate these centrally in each instance with a serum. Moreover, by reason of the unequal height of the outlet tips of the multipipette, the result is a nonuniform distribution on the object stage. In order to prevent the serum from running from one cell culture to the next, it is accordingly known to cover the object stage with a protective layer, preferably of teflon, with a multiplicity of recesses in which a cell culture is situated in each instance. The use of object stages covered with a protective layer does indeed facilitate the handling and protection against running, but the application of the protective layer to the object stage cannot be undertaken by the test laboratories themselves and for this reason it is necessary to use expensive object stages which have already been coated. Object stages which are commercially available and which are provided with a protective layer exhibit in addition the disadvantage that their protective layer reacts with sensitivity to certain cleaning fluids, more especially acetone, and can therefore only be used to a restricted extent.
The application of object stages which are not covered with a protective layer sets narrow limits for application in the case of the abovementioned investigation, since the spacings of the cell cultures applied to the object stage must be chosen to be very large in order to prevent running, so that the application of object stages of such a kind is uneconomic.
From U.S. Pat. No. 377,699, an arrangement is indeed known which permits a regular application of a liquid in very small quantities to an object stage; however, since no protective layer is employed on the object stage, in this instance the problem of running is not corrected. The application of the liquid takes place in this instance in depressions which are upwardly open. Since the individual drops accordingly fall on to the object stage from a determined height, running of the drops cannot be ruled out. This is all the more possible, as the minimum quantity of fluid which can be applied with this device exceeds 25 .mu.L.
The object of the invention is to indicate a process for the simultaneous application of a multiplicity of liquid samples to an object stage, in which the liquid samples can be disposed at a small spacing beside one another on the object stage, without running taking place between them. A further object of the invention is to indicate an apparatus for carrying out the process, which can be handled in a simple manner and which can be produced cheaply and which is adapted to the particular requirements of the laboratory investigation. A further object consists in indicating an object stage which is prepared in such a manner that a sequential investigation of sera can be carried out quickly, accurately and reliably.
The object of the invention is fulfilled by the invention indicated in the claims. Advantageous refinements of the invention are indicated in subclaims, the description and the drawings.
The process according to the invention permits a very rapid and reliable application of a multiplicity of liquid samples to an
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Hoft Alois
Noss Gerhard
Feix Donald C.
Freiburger T. M.
Odar H. A.
Scott Samuel
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