Spectrophotometric method and apparatus

Optics: measuring and testing – By dispersed light spectroscopy – Utilizing a spectrometer

Reexamination Certificate

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Reexamination Certificate

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07576855

ABSTRACT:
Disclosed is a spectrophotometry for a spectral transmission measurement of an extremely small volume of sample solution. A sampler15is formed of a flat plate member formed with a sample-holding hole152which penetrates the flat plate member in its thickness direction and has a reverse truncated cone shape, and a target sample solution is dropped into the sample-holding hole152of the sampler15positioned approximately horizontally and held in the hole152based on a surface tension of the sample solution in the form of a droplet S. Then, a measurement light is emitted to the held droplet S from directly above. A transmitted light getting out of the droplet S downward is introduced to a diffraction grating19through a window plate17and a slit18, and dispersed in a wavelength dependent manner. The resulting light with dispersed wavelength components is detected by a multichannel detector20approximately simultaneously.

REFERENCES:
patent: 4682890 (1987-07-01), de Macario et al.
patent: 5115133 (1992-05-01), Knudson
patent: 7316899 (2008-01-01), McDevitt et al.
patent: 2002/0149775 (2002-10-01), Mori et al.
patent: 05-302893 (1993-11-01), None
NanoDrop products: NanoDrop 1000 Overview,Thermo Fischer Scientific, © 2008, URL: http://www.nanodrop.com
d-1000-overview.html.

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