Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2002-08-06
2009-08-18
Collins, Cynthia (Department: 1638)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S468000, C536S023400, C800S278000
Reexamination Certificate
active
07575898
ABSTRACT:
A nucleic acid molecule is disclosed as containing a first nucleic acid sequence comprising a nucleotide sequence that encodes γ-zein protein, or a fragment thereof capable of directing and retaining a protein towards the endoplasmic reticulum (ER) of a plant cell; a second nucleic acid sequence containing a nucleotide sequence that encodes an amino acid sequence that is specifically cleavable by enzymatic or chemical means; and a third nucleic acid sequence containing the nucleotide sequence that encodes a peptide or protein of interest. Methods of using this nucleic acid molecule for transforming host plant cells and producing the peptide or protein of interest are also disclosed.
REFERENCES:
patent: 4215040 (1980-07-01), Hager
patent: 5948682 (1999-09-01), Moloney et al.
patent: 6642437 (2003-11-01), Lemaux et al.
patent: 2005/0244924 (2005-11-01), Wagner et al.
patent: WO 96/21029 (1996-07-01), None
patent: WO 00/40738 (2000-07-01), None
patent: WO 02/086077 (2002-10-01), None
Ofoghi H. et al. Cloning and expression of human calcitonin genes in transgenic potato plants. Biotechnology Letters, vol. 22, No. 7, Apr. 2000, pp. 611-615.
McKee C. et al. Production of biologically active salmon calcitonin in the milk of transgenic rabbits. Nat Biotechnol. Jul. 1998;16(7):647-51.
Torrent M. et al. Lysine-rich modified gamma-zeins accumulate in protein bodies of transiently transformed maize endosperms. Plant Mol Biol. May 1997;34(1):139-49.
Geli M.I. et al. Two Structural Domains Mediate Two Sequential Events in gamma-Zein Targeting: Protein Endoplasmic Reticulum Retention and Protein Body Formation. Plant Cell. Dec. 1994;6(12):1911-1922.
Cramer C.L. et al. Transgenic plants for therapeutic proteins: linking upstream and downstream strategies. Curr Top Microbiol Immunol. 1999;240:95-118. Review.
Conrad U. et al. Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol Biol. Sep. 1998;38(1-2):101-9. Review.
Reichel C. et al. Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc Natl Acad Sci U S A. Jun. 11, 1996;93(12):5888-93.
Ludevid Mugica et al., Sequence 7, Publication No. US20040005660A1, Jan. 8, 2004.
Bevan, M. 1984,Nucleic Acids Research12:8711-8721.
Conrad, U. and Fiedler, U. 1998,Plant Mol. Biol. 38:101-109.
Dilsen et al., 2000,Appl. Microbiol. Biotechnol. 54:361-369.
Düring et al., 1990,Plant Mol. Biol. 15:281-293.
Fischer, R. and Emans, N. 2000,Transgenic Research9:279-299.
Haq et al., 1995,Science268:714-715.
Herman, E.M. and Larkins, B.A. 1999,Plant Cell11:601-613.
Hong et al., 2000,Biochem. Biophys. Res.Com. 267:362-367.
Kogan et al., 2001,J. Mol. Biol. 00:1-7.
Logemann et al., 1987,Anal. Biochem. 163:16-20.
Ludevid et al., 1984,Plant Mol. Biol. 3:277-234.
Ma et al., 1998,Nat. Med. 4:601-606.
Ma, J.K. and Hein, M.B. 1995,Plant Physiol. 109:341-346.
McCormick et al., 1999,Proc. Natl. Aca. Sci. USA96 (2):703-708.
McKee et al., 1998,Nature Biotechnology16:647-651.
Merli et al., 1996,Protein Express. Purif. 7:347-354.
Moore et al., 1991,J. Cell Biol. 112:589-602.
Munro, S. and Pelham, H.R.B. 1987,Cell48:899-907.
Müntz, K. 1998,Plant Mol. Biol. 38:77-99.
Parmenter et al., 1995,Plant Mol. Biol. 29:1167-1180.
Ray et al., 1993,Bio/Technology11:64-70.
Reichel et al., 1996,Proc. Natl. Acad. Sci. USA93:5888-5893.
Staub et al., 2000,Nature Biotechnology18:333-338.
Stöger et al., 2000,Plant Mol. Biol. 42:583-590.
Takahashi et al., 19997,Peptides18:439-444.
Torrent et al., 1986, Plant Mol. Biol. 7:393-403.
Torres et al., 1999,Transgenic Research8:441-449.
Verch et al., 1999,J. Immunol. Methods220:69-75.
Vitale, A. and Denecke, J. 1999,Plant Cell11:615-628.
Wandelt et al., 1992,Plant J. 2:181-192.
Yamamoto et al., 2001,EMBO J. 20(12):3082-3091.
Cudd et al., 1995,J. Pharm. Sci. 84:717-719.
Eipper et al., 1992,Annu. Rev. Neurosci. 15:57-85.
Vitale et al., 1993,J. Exp. Bot.44:1417-1444.
Torrent et al., 1994,Planta192:512-518.
Azria et al., 1995Tissue Int. 57(6):405-408.
Cramer et al., 1999Curr. Top. Microbiol. Immunol. 240:95-118.
Reginster, J.Y. 1993Am. J. Med. 95(5A):44S-47S.
Silverman, S.L. 1997Am. J. Med. Sci. 313(1):13-16.
Yabuta et al., 1995Appl. Microbiol. Biotechnol. 42(5):703-708.
Geli et al., 1994,Plant Cell6:1911-1922.
Ems-McClung et al., 2002Plant Science162(1):131-141.
Alvarez et al., 1998Planta(Berlin) 205(3):420-427.
Philip et al., 1998Plant Science137(2):191-204.
Torrent et al., 1997Plant Molecular BiologyGeli et al., 1994,Plant Cell6:1911-1922 (34(1):139-149.
Goytia, E. et al., “Production of Plum Pox Virus HC-Pro Functionally Active for Aphid Transmission in a Transient-Expression System”,Journ. of General Virol., 37:3413-3423 (2006).
Shukla et al., “Zein: The Industrial Protein from Corn”,Industrial Crops and Product, 13:171-192 (2001).
International Search Reports dated Mar. 21, 2006.
International Search Reports dated Apr. 21, 2006.
International Search Reports dated Nov. 23, 2007.
International PCT Invitation with Annex Search Reports dated Jul. 31, 2007.
Altschuler et al., “The N- and C-Terminal Regions Regulate the Transport of Wheat γ-Gliadin through the Endoplasmic Reticulum in Xenopus Oocytes,”The Plant Cell, 5:443-450 (Apr. 1993).
Cameron-Mills, “The Structure and Composition of Protein Bodies Purified from Barley Endosperm by Silica Sol Density Gradients”,Carlsberg Res. Commun, 45:557-576 (1980).
Kim et al., “Zein Protein Interactions, Rather Than the Asymmetric Distribution of Zein mRNAs on Endoplasmic Reticulum Membranes, Influence Protein Body Formation in Maize Endosperm,”The Plant Cell, 14:655-672, Mar. 2002.
Mainieri et al., “Zeolin. A New Recombinant Storage Protein Constructed Using Maize γ-Zein and Bean Phaseolin,”Plant Physiol., 136:3447-3456 (2004).
Miflin, et al., “The Development of Protein Bodies in the Storage Tissues of Seeds: Subcellular Separations of Homogenates of Barley, Maize, and Wheat Endosperms and of Pea Cotyledons,”Journ. of Exp. Botany, 32:199-219 (1981).
Richard et al., “Transport and Deposition of Cereal Prolamins”,Plant Physiol. Biochem., 34:237-243 (1996).
Rosenberg et al., “Wheat (Wheat (Triticum aestivumL.) γ-Gliadin Accumulates in Dense Proteins Bodies within the Endoplasmic Reticulum of Yeast,”Plant Physiol., 102:61-69 (1993).
Sojikul et al., “A Plant Signal Peptide-Hepatitis B Surface Antigen Fusion Protein with Enhanced Stability and Immunogenicity Expressed in Plant Cells,” PNAS, 100(5):2209-2214 (2003).
Takagi et al., “A Rice-Based Edible Vaccine Expressing Multiple T Cell Epitopes Induces Oral Tolerance for Inhibition of Th2-Mediated 1gE Responses,”PNAS, 102(48):17525-17530 (2005).
Wallace et al., “Aggregation of Lysine-Containing Zeins into Protein Bodies inXenopus Oocytes,” Science, 240:662-664 (1988).
Ludevid Mugica Maria Dolores
Ramassamy Sabine
Torrent Quetglas Margarita
Collins Cynthia
ERA Plantech, S.L.
Husch Blackwell Sanders Welsh & Katz
LandOfFree
Production of peptides and proteins by accumulation in plant... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of peptides and proteins by accumulation in plant..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of peptides and proteins by accumulation in plant... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4098126