Fragment complementation assays for G-protein-coupled...

Details Fragment complementation assays for G-protein-coupled... Fragment complementation assays for G-protein-coupled...

2004-09-23

2009-02-10

Ulm, John D (Department: 1649)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007200, C435S007210, C435S069700, C436S501000, C536S023400

Reexamination Certificate

active

07488583

ABSTRACT:
The invention provides a method of screening a candidate drug, a compound library or a biological extract to identify activators or inhibitors of G-protein-coupled receptors or G-protein-coupled pathways, comprising: (A) using a fluorescent protein fragment complementation assay to construct an assay for one or more steps in a G-protein-coupled pathway; (B) testing the effects of the candidate drugs, compound library, or biological extract on the receptor or pathway of interest; and (C) using the results of the screening to identify specific agents that activate or inhibit the receptor or pathway of interest. The invention also provides a method for identifying a drug lead that modulates the activity of a G-protein-coupled pathway using a fluorescent protein fragment complementation assay. The method of the invention is used to identify agonists, antagonists, activators or inhibitors of G-protein coupled receptors or G-protein-coupled pathways.

REFERENCES:
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patent: 6294330 (2001-09-01), Michnick et al.
patent: 6893827 (2005-05-01), Palmer et al.
patent: 7166424 (2007-01-01), Michnick et al.
Remy et al. Clonal Selection in vivo Quantitation of Protein Interactions with Protein-Fragment Complementation Assays. May 1999, P.N.A.S. 96:5394-5399.

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