Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...
Reexamination Certificate
2006-04-24
2008-11-04
Epperson, Jon D. (Department: 1639)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Amino acid sequence disclosed in whole or in part; or...
C530S328000, C530S345000
Reexamination Certificate
active
07445784
ABSTRACT:
This invention characterizes the specific peptide fragment derived from specially prepared zinc charged fetuin and a method of preparation thereof, wherein the fragment was found to contain an apoptosis-inducing activity. Specifically, the amino acid sequence of this peptide is His Thr Phe Ser Gly Val Ala Ser Val Glu (SEQ ID NO: 1). The peptide induced apoptosis in LNCaP (prostate cancer) and HT-29 (colon cancer) cells without affecting CCD 18 Co (normal colon) cells. The in vitro tissue culture study demonstrated that the peptide is more potent than the parent molecule (full-length zinc charged fetuin) in inducing apoptosis. The peptide has a LD50of 0.3-0.4 μM, while the LD50for zinc-charged fetuin is 3-10 μM.
REFERENCES:
patent: 7193040 (2007-03-01), Tsai
patent: 2003/0050446 (2003-03-01), Ramakrishnan
patent: 2005/0277594 (2005-12-01), Tsai
patent: 2006/0258595 (2006-11-01), Tsai
patent: WO 98/30583 (1998-07-01), None
Cianfrocca et al., 2006, British Journal of Cancer, 1-6.
Yu et al., 2001, Embryonic apoptosis-inducing protiens exhibited anticancer activity in vitro and in vivo, Anticancer Research, 21(3B): 1839-1856.
Yu et al., 2001, Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models, Cancer Letters, 166: 173-184.
Auberger et al., 2006, Current Cancer Drug Targets, 6: 271-294.
Dziegielewska et al., 1990, The Complete cDNA and Amino Acid Sequence of Bovine Fetuin, The Journal of Biological Chemistry, 265(8): 4354-4357.
Brown et al., 1992, The nucleotide and deduced amino acid structures of sheeep and pig fetuin, Eur. J. Biochem., 205: 321-331.
Krammer, “Apoptosis in the APO-1 system” Apoptosis, the Molecular Basis for Cell Death (1991), CSHL Press, pp. 87-99.
Michaelson, “Cell selection in development”Biol. Review., Cambridge Philos. Soc. 62:115 (1987).
Wyllie, “Cell death, significance in apoptosis”, Int. Rev. Cytol. 68:251 (1980).
Cope, “Apoptosis, the Molecular Basis for Cell Death” (1991), CSHL Press, p. 61.
Yang, “Human alpha-2HS glycoprotein/bovine fetuin homologue in mice: identification and developmental regulation of teh gene” Biochim. Biophys. Acta, 1130:149-156 (1992).
Von Bulow, “Human alpha-2HS glycoprotein in colloid and parenchymal cells in human fetal pituitary gland”, Histochemistry, 99:13-22 (1993).
Dziegielewska, Fetuin 1995, R.G. Landes Co., pp. 16-17.
Lin, “In vitro apoptosis of the human hepatoma cell line is iunduced by TGFbeta1”, Cancer Res. 52:385 (1992).
Kawakawi, “Cachectin/TNF kills or inhibits the differentiation of 3T3-L1 cells according to developmental stage”, J. Cell. Physiol., 138:1 (1989).
Wiley, “Identification and characterization of a new member of the TNF family that induces apoptosis”, Immunity, 3:673 (1995).
McGrath, Clinics in Plastic Surgery, 17:421 (1993).
Ambryx Biotechnology, Inc.
Epperson Jon D.
Steele Amber D.
Trojan Law Offices
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