Manipulation of the phenolic acid content and digestibility...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide

Reexamination Certificate

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C800S287000, C800S298000, C800S320000

Reexamination Certificate

active

07453023

ABSTRACT:
Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I fromAspergillus, preferablyA. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase fromTrichoderma, preferablyT. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

REFERENCES:
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patent: 6602700 (2003-08-01), Li et al.
patent: 2 301 103 (1996-11-01), None
Kroon P. et al. Biochemical Society Transactions, 1998; vol. 26; p. S167.
*Darnowski, D. W. et al., << A soybean lectin-GFP fusion labels the vacuoles in developingArabidopsis thalianaembryos, >> Plant Cell Reports, vol. 20, No. 11, pp. 1033-1038, May 2002.
*De Vries. R. P. et al., “The faeA genes fromAspergillus nigerandAspergillus tubingensisencode ferulic acid esterases involved in degradation of complex cell wall polysaccharides,” Applied and Environmental Microbiology, vol. 63, No. 12, Dec. 1997 pp. 4638-4644, XP00203731.
*Garcia-Conesa, Maria-Teresa et al., “A cinnamoyl esterase fromAspergillus nigercan break plant cell wall cross-links without release of free diferulic acids.” European Journal of Biochemistry, vol. 266, No. 2, Dec. 1999, pp. 644-652, XP002203732.

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