Peroxide-driven cytochrome P450 oxygenase variants

Details Peroxide-driven cytochrome P450 oxygenase variants Peroxide-driven cytochrome P450 oxygenase variants

2002-04-16

2008-12-16

Pak, Yong D (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S025000, C435S069100, C435S071100, C435S440000, C435S006120, C536S023200, C536S063000, C536S063000

Reexamination Certificate

active

07465567

ABSTRACT:
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.

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