Selective binding complementary oligonucleotides

Details Selective binding complementary oligonucleotides Selective binding complementary oligonucleotides

1995-10-04

1999-06-15

Houtteman, Scott W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 266, 536 243, C07H 2104

Patent

active

059123400

ABSTRACT:
In a matched pair of oligonucleotides (ODNS) each member of the pair is complementary or substantially complementary in the Watson Crick sense to a target sequence of duplex nucleic acid where the two strands of the target sequence are themselves complementary to one another. The ODNs include modified bases of such nature that the modified base forms a stable hydrogen bonded base pair with the natural partner base, but does not form a stable hydrogen bonded base pair with its modified partner. This is accomplished when in a hybridized structure the modified base is capable of forming two or more hydrogen bonds with its natural complementary base, but only one hydrogen bond with its modified partner. Due to the lack of stable hydrogen bonding with each other, the matched pair of oligonucleotides have a melting temperature under physiological or substantially physiological conditions of approximately 40.degree. C. or less. However each of the matched ODN pair of the invention forms a substantially stable hybrid with the target sequence in each strand of the duplex nucleic acid. The hybrids of target duplex nucleic acids formed with the ODN pairs of the invention are useful for gene mapping and in diagnostic and therapeutic applications.

REFERENCES:
Scheit et al. Studia Biophysica vol. 55, No. 1, 1976. pp. 21-27.
Inoue et al. Chemical Abstracts vol. 108, No. 21, 1988. p. 752, col. 1. vol. 012, No. 139 (see abstract).
Case-Green et al. Nucleic Acids Research, vol. 22, No. 2, 1994. pp. 131-136.
Martin et al. Nucleic Acids Research, vol. 13, No. 24, 1985. pp. 8927-8938.
Chollet et al. Nucleic Acids Research, vol. 16, No. 1, 1988. pp. 305-317.
Kuimelis et al. Nucleic Acids Research, vol. 22, No. 8, 1994. pp. 1429-1436.
Ishikawa et al. Chemical Abstracts, vol. 116, No. 13, 1992. pp. 949. col. 2.
Newman et al. Biochemistry, vol. 29, No. 42, 1990. pp. 9891-9901.
Richardson et al. Journal of the American Chemical Society, vol. 113, No. 13, 1991. pp. 5109-5111.
Woo J et al. Nucleic Acids Research, vol. 24, No. 13, 1996. pp. 2470-2475.2.
Strobel, S.A., et al. (1991) Science, 254:1639.
Weinstock, P., et al. (1990) Nucl. Acids Res., 18:4207.
Roca, A.I., et al. (1990) Rev. Biochem. Mol. Biol., 25:415.
Robins et al.(1982) Can. J. Chem., 60:554.
Robins et al.(1983) J. Org. Chem., 48:1854.
Meyer, et al., (1989) J. Am. Chem. Soc., 111:8517.
Kobayashi (1973) Chem. Phar. Bull., 21:941-951.
Sonveaux (1986) Bioorganic Chemistry, 14:274-325.
Jones (1984) "Oligonucleotide Synthesis, a Practical Approach", M.J. Gait, Ed., IRL Press, P23-34.
Langer et al. (1981) Proc. Natl. Acad. Sci. USA, 78:6633-6637. "Nucleic Acid Hybridisation, a Practical Approach", Hames and Higgins, Eds., IRL Press, 1985.
Gall and Pardue, (1969) Proc. Natl. Acad. Sci., U.S.A., 63:378-383.
John et al., (1969) Nature, 223:582-587.
"Physical Biochemistry", Freifelder, D., W.H. Freeman & Co., 1982, pp. 537-542.
Tijssen, P., (1985) "Practice and theory of Enzyme Immunoassays, Laboratory Techniques in Biochemistry and Molecular Biology", Burdon, R.H., van Knippenberg, P.H., Eds, Elsevier, pp. 9-20.
Connolly, et al., (1989) Nucleic Acids Res.,17:4957-4974.
Fathi, et al. (1990) Tetrahedron Lett., 31:319-322.
Sinha, et al. (1984) Nucleic Acids Research, 12:4539.
Alul, et al. (1991) Nucleic Acids Res., 19:1527-1532.
Atkinson, T. and Smith, M. (1984) "Oligonucleotide Synthesis, a Practical Approach", M. Gait, Ed., IRL Press, Washington, D.C., pp. 35-81.

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