Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Reexamination Certificate
2002-09-04
2008-08-12
Bertoglio, Valarie (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
C435S373000, C435S375000, C435S394000
Reexamination Certificate
active
07410798
ABSTRACT:
This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
REFERENCES:
patent: 5166065 (1992-11-01), Williams et al.
patent: 5332672 (1994-07-01), Conover et al.
patent: 5405772 (1995-04-01), Ponting
patent: 5453357 (1995-09-01), Hogan
patent: 5523226 (1996-06-01), Wheeler
patent: 5583016 (1996-12-01), Villeponteau et al.
patent: 5639618 (1997-06-01), Gay
patent: 5843780 (1998-12-01), Thomson
patent: 5914268 (1999-06-01), Keller et al.
patent: 5922597 (1999-07-01), Verfaillie et al.
patent: 5942435 (1999-08-01), Wheeler
patent: 5968829 (1999-10-01), Carpenter
patent: 6800480 (2004-10-01), Bodnar et al.
patent: 7005252 (2006-02-01), Thomson
patent: 2005/0148070 (2005-07-01), Thomson et al.
patent: 199912771 (1999-05-01), None
patent: 0695 351 (1999-12-01), None
patent: 2744133 (1997-08-01), None
patent: WO 96/17627 (1996-06-01), None
patent: WO 97/28253 (1997-08-01), None
patent: WO 97/30151 (1997-08-01), None
patent: WO 97/47734 (1997-12-01), None
patent: WO 98/30679 (1998-07-01), None
patent: WO 98/43679 (1998-10-01), None
patent: WO 99/42122 (1999-08-01), None
patent: WO 01/51616 (2001-07-01), None
Dvorak et al. Stem Cells (2005) 23:1200-1211.
Zhang et al. Nature biotechnology (Dec. 2001) 19:1129-1133.
Baribault, H., et al., “Embryonic Stem Cell Culture and Gene Targeting in Transgenic Mice”, Mol. Biol. Med. 6:481-492 (1989).
Berger, C., et al., “Self Renewal of Embryonic Stem Cells in the Absence of Feeder Cells and Exogenous Leukaemia Inhibitory Factor”, Growth Factors, 14:145-159 (1997).
Bongso, A., et al., “Improved Quality of Human Embryos When Co-Cultured with Human Ampullary Cells”, Hum Reprod., 4:706-713 (1989).
Bradley, A., et al., “Modifying the Mouse: Design and Desire”, Biotechnology, 10:534-539 (1992).
Evans, M., et al., “Establishment in Culture of Pluripotential Cell from Mouse Embryos”, Nature, 292:154-156 (1981).
Gardner, D., et al., “Culture and Transfer of Human Blostocysts Increases Implantation Rates and Reduces the Need for Multiple Embryo Transfers”, Fertil. Steril. 69:84-88 (1998).
Gendall, A., et al., “Isolation and Characterization of a Leukemia Inhibitory Factor-Independent Embryonic Stem Cell Line”, Int. J. Biochem Cell Biol., 29(5):829-840 (1997).
Gendron, R., et al., “Induction of Embryonic Vasculogenesis by bFGF and LIF in Vitro and in Vivo”, Developmental Biology, 177:332-346 (1996).
GibcoBrl Life Technologies Catalogue and Ref. Guide, pp. 1-2 through 1-4, 1-94 and 1-95 (1993).
Keller, G., “In Vitro Differentiation of Embryonic Stem Cells”, Cell Biology, 7:862-869 (1995).
Koshimizu, U., et al., “Functional Requirement of gp130-mediated Signaling for Growth and Survival of Mouse Primordial Germ Cells In Vitro and Derivation of Embryonic Germ (EG) Cells”, Development, 122:1235-1242 (1996).
Koshimizu, U., et al., “Rapid Communication Retinoic Acid Is a Potent Growth Activator of Mouse Primordial Germ Cells in Vitro”, Developmental Biology, 168:683-685 (1995).
Matsui, Y., et al., “Derivation of Pluripotential Embryonic Stem Cells from Murine Primordial Germ Cells in Culture”, Cell, 70:841-847 (1992).
Nichols, J., et al., “Establishment of Germ-line-Competent Embryonic Stem (ES) Cells Using Differentiation Inhibiting Activity”, Development, 110:1341-1348 (1990).
Nichols, J., et al., “Derivation of Germline Competent Embryonic Stem Cells with a Combination of Interleukin-6 and Soluble Interleukin-6 Receptor”, Experimental Cell Research, 215:237-239 (1994).
Pease, S., et al., “Isolation of Embryonic Stem (ES) Cells in Media Supplemented with Recombinant Leukemia Inhibitory Factor (LIF)”, Developmental Biology, 141:344-352 (1990).
Pedersen, R., “Studies of In Vitro Differentiation with Embryonic Stem Cells”, Reprod. Fertil. Dev., 6:543-52 (1994).
Robertson, E., “Derivation and Maintenance of Embryonic Stem Cell Cultures”, Methods in Mol. Bio., 75:173-184 (1997).
Rose, T., et al., “Oncostatin M (OSM) Inhibits the Differentiation of Pluripotent Embryonic Stem Cells In Vivo”, Cytokine, 6(1):48-54 (1994).
Shamblott, M., et al., “Derivation of Pluripotent Stem Cells from Cultured Human Primordial Germ Cells”, Proc. Natl. Acad. Sci. USA, 95:13726-13731 (1998).
Smith, A., et al., “Inhibition of Pluripotential Embryonic Stem Cell Differentiation by Purified Polypeptides”, Nature, 336:668-690 (1998).
Thomson, J., et al., “Embyronic Stem Cell Lines Derived from Human Blastocysts”, Science, 282:145-47(1998).
Thomson, J., et al., “Isolation of a Primate Embryonic Stem Cell Line”, Proc. Natl. Acad. Sci. USA, 92:7844-7848 (1995).
Thomson, J., et al., “Primate Embryonic Stem Cells”, Current Topics in Developmental Biology, 38:133-165 (1998).
Worrall, D., et al., “A Carrot Leucine-Rich-Repeat Protein That Inhibits Ice Recrystallization”, Science, 282:115-117 (1998).
Williams, R., et al., “Myeloid Leukaemia Inhibitory Factor Maintains the Developmental Potential of Embryonic Stem Cells”, Nature, 336:684-687 (1988).
Berger,C., et al., “Self Renewal of Embryonic Stem Cells in the Absence of Feeder Cells and Exogenous Leukaemia Inhibitory Factor”, Growth Factors, 14:145-159 (1997).
Keller, G., “In Vitro Differentiation of Embryonic Stem Cells”, Current Opinion in Cell Biology, 7:862-869 (1995).
Koshimizu, U., et al., “Rapid Communication: Retinoic Acid is a Potent Growth Activator of Mouse Primordial Germ Cells in Vitro”, Developmental Biology, 168:683-685 (1995).
Nichols, J., et al., “Derivation of Germline Competent Embryonic Stem Cells with a Combination of Interleukin-6 and Soluble Interleukin-6 Receptor”, Experimental Cell Res., 215:237-239 (1994).
Wenk, J., et al., “Glycolipids of Germ Cell Tumors: Extended Globo-Series Glycolipids are a Hallmark of Human Embryonal Carcinoma Cells”, Int. J. Cancer, 58:108-115 (1994).
Xu, C., et al., “Feeder-free growth of undifferentiated human embryonic stem cells,” Nat Biotechnol 19:971 (2001).
Carpenter Melissa K.
Gold Joseph D.
Mandalam Ramkumar
Xu Chunhui
Bertoglio Valarie
Geron Corporation
Mittler E. Stewart
LandOfFree
Culture system for rapid expansion of human embryonic stem... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Culture system for rapid expansion of human embryonic stem..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Culture system for rapid expansion of human embryonic stem... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4008831