Multiplexing assays for analyte detection

Details Multiplexing assays for analyte detection Multiplexing assays for analyte detection

2005-06-10

2008-09-09

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C530S300000

Reexamination Certificate

active

07422855

ABSTRACT:
High density multiplexing assays for proteins, nucleic acids, and other molecules are described based upon using mass spectrometry detection. A solid-state support assay format is utilized, whereby, the bead carries both a coding molecule to track the sample or analyte and the molecule(s) necessary to perform an affinity-based assay. After the necessary reactions are complete, all of the components, e.g., coding molecule, binding molecule, analyte, and/or label are dissociated for analysis in a mass spectrometer. Various means of performing detection for the assays include different types of mass spectrometers with different types of sampling systems, including MALDI-TOF and ESI.

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