Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2004-08-09
2008-09-02
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S006120, C435S071100
Reexamination Certificate
active
07419801
ABSTRACT:
Vectors, host cells, and methods are provided for the production of proteins in yeast. The vectors generally contain a selection gene, a yeast 2 micron sequence, and a polynucleotide encoding a polypeptide, where the polynucleotide is operably linked to promoter, and where the polynucleotide contains one or more yeast-preferred codons. Host cells are cultured under conditions where, after an initial batch phase, oxygen concentration is kept high and glucose feed is regulated so that the yeast cells stay in respiratory metabolism.
REFERENCES:
patent: 4752576 (1988-06-01), Brake et al.
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 4837148 (1989-06-01), Cregg
patent: 4839283 (1989-06-01), Kawasaki et al.
patent: 4929555 (1990-05-01), Cregg et al.
patent: 4940661 (1990-07-01), Etcheverry et al.
patent: 5010003 (1991-04-01), Chang et al.
patent: 5013652 (1991-05-01), Strausberg et al.
patent: 5922569 (1999-07-01), Heim et al.
patent: 6068994 (2000-05-01), Barr
patent: 6103500 (2000-08-01), Innis et al.
patent: 6204012 (2001-03-01), Hellmuth et al.
patent: 0 284 044 (1988-09-01), None
patent: 73654 (1990-12-01), None
patent: WO 84/04538 (1984-11-01), None
patent: WO 02/50287 (2002-06-01), None
Amiali et al. High nisin Z production byLactococcus lactisUL719 in whey permeate with aeration. World Journal of Microbiology & Biotechnology. 14: 887-894. 1998.
du Preez, J.C., FEMS Yeast Research, vol. 1, 233-240, (2001).
European Search Report dated Jan. 9, 2007.
Kaslow and Shiloach, Bio/Technology, vol. 12, 494-499 (1994).
Noronha, S.B., Journal of Individual Microbiology & Biotechnology, vol. 20, 192-199 (1998).
O'Connor, G.M., Biotechnology and Bioengineering, vol. 39:(3), 293-304 (1992).
Sabin, et al., “High-Level Expression and In Vivo Processing of Chimeric Ubiquitin Fusion Proteins inSaccharomyces cerevisiae”, Bio/Technology, vol. 7, Jul. 1989, pp. 705-709.
Bennetzen and Hall, “Codon Selection in Yeast”, The Journal of Biological Chemistry, vol. 257, No. 6, pp. 3026-3031, Mar. 1982.
Tamer and Chisti, Production and Recovery of Recombinant Protease Inhibitor α1-Antitrypsin, Enzyme and Microbial Technology, vol. 29, pp. 611-620, 2001.
Russell, et al., “Nucleotide Sequence of the Yeast Alcohol Dehydorgenase II Gene”, The Journal of Biological Chemistry, vol. 258, No. 4, pp. 2674-2682, 1983.
Barr, et al., “Yeast Genetic Engineering”, Chp. 6, Butterworths, 1989.
Broach, et al., “Transformation in Yeast: Development of a hybrid Cloning Vector and Isolation of theCAN1 Gene”, Gene, vol. 8, pp. 121-133, 1979.
Pearson and Lipman, “Improved Tools for Biological Sequence Comparison”, Proceedings of the National Academy of Science, vol. 85, pp. 244-2448, Apr. 1988.
Tuite, et al., “Regulated High Efficiency Expression of Human Interferon-Alpha inSaccharomyces cerevisiae”, The EMBO Journal, vol. 1, No. 5, pp. 603-608, 1982.
Kurachi, et al., “Cloning and Sequence of cDNA Coding for α1-Antitrypsin”, Proceedings of the National Academy of Science (Biochemistry), vol. 78, No. 11, pp. 6826-6830, Nov. 1981.
Kurjan and Herskowitz, “Structure of a Yeast Pheromone Gene (MFα): A Putative α-Factor Precursor Contains Four Tandem Copies of Mature α-Factor”, Cell., vol. 30, pp. 933-943, Oct. 1982.
Zakian and Scott, “Construction, Replication, and Chromatin Structure of TRP1 R1 Circle, a Multiple-Copy Synthetic Plasmid Derived fromSaccharomyces cerevisiaeChromosomal DNA”, Molecular and Cellular Biology, pp. 221-232, Mar. 1982.
Dayhoff, et al., Atlas of Protein Sequence and Structure, National Biomedical Research Foundation, vol. 5, Suppl.3, “A Model of Evolutionary Change in Proteins”, pp. 345-358, 1979.
Hein, “Unified Approach to Alignment and Phylogenies”, Methods in Enzymology, vol. 183, pp. 626-645, 1990.
Higgins, et al., “Fast and Sensitive Multiple Sequence Alignments on a Microcomputer”, Cabios Communications, vol. 5, No. 2, pp. 151-153, 1989.
Jones, “Vacuolar Proteases in YeastSaccharomyces cerevisiae”, Methods In Enzymology, vol. 85, Chp 31, pp. 372-386, 1990.
Kurtz, et al., “Integrative Transformative ofCandida albicans, Using a ClonedCandida ADE2Gene”, Molecular and Cellular Biology, vol. 6, No. 1, pp. 142-149, Jan. 1986.
Kunze, et al., “Transformation of the Industrially Important YeastsCandida maltosaandPichia guilliermondii”, Journal of Basic Microbiology. vol. 25, pp. 141-144, 1984.
Myers, et al. “Optimal Alignments in Linear Space”, Cabios Communications, vol. 4, No. 1, pp. 11-17, 1988.
Robinson, “Comparison of labeled Trees with Valency Three”, Journal of Combinational Theory, vol. 11, pp. 105-119, 1971.
Saitou, “The Neighbor-Joining Method: A new Method for Reconstructing Phylogenetic Trees”, Molecular Biology and Evolution, pp. 406-425, 1987.
Wilbur, et al., “Rapid Similarity Searches of Nucliec Acid and Protein Data Banks”, Proceedings of the National Academy of Science, USA, vol. 80, pp. 726-730, Biochemistry, Feb. 1983.
Zou, et al., “Mapsin, a Serpin with Tumor-Suppressing Activity in Human Mamary Epithelial Cells”, Science, vol. 263, pp. 526-529, Jan. 28, 1994.
Gleeson, et al., “Transformation of theMethylotrophic YeastHansenula polymorpha”, Journal of General Microbiology, vol. 132, pp. 3459-3465, 1986.
Roggenkamp, et al., “Transformation of the Methylotrophic YeastHansenula polymorphaby Autonomous Replication and Integration Vectors”, Mol. Gen. Genet., 220, pp. 302-308, 1986.
Das, et al., et al., “Transformation ofKluyveromyces fragilis”, Journal of Bacteriology, vol. 158, No. 3, pp. 1165-1167, Jun. 1984.
De Louvencourt, et al., “Transformation ofKluyveromyces fragilis”, Journal of Bacteriology, vol. 154, No. 2, pp. 737-742, May 1983.
Van Den Berg, et al., “Kluyveromyces as a host of rHerterologous Gene Expression: Expression and Secretion of Prochymosin”, Bio/Technology, vol. 8, pp. 135-140, Feb. 1990.
Cregg, et al., “Pichia pastorisas a Host System for Transformations”, Molecular and Cellular Biology, vol. 5, No. 12, pp. 3376-3385, 1985.
Hinnen, et al., “Transformation of Yeast”, Proceedings of the National Academy of Science, USA, Genetics, vol. 75, No. 4, pp. 1929-1933, Apr. 1978.
Ito, et al., “Transformation of Intact Yeast Cells Treated with Alkali Cations”, Journal of Bacteriology, vol. 153, No. 1, 1983.
Davidow, et al., “Integrative Transformation of the YeastYarrowia lipolytica”, Current Genetics, vol. 10, pp. 39-48, 1985.
Gaillardin, et al., “Integrative transformation of the YeastYarrowia lipolytica”, Current Genetics, vol. 10, pp. 49-58, 1985.
Winkler, “Time-Profiling and Environmental Design in Computer -Controlled Fermentation and Enzyme Production”, Genetically-Engineered Proteins and Enzymes from Yeast : Production Control, editor: Alan Wiseman, Ellis Horwood Limited, Chp. 4 and 5, 1991.
Schiestl, et al., “High Efficiency Transformation of Intact Yeast Cells Using Single Stranded Nucleic Acids as a Carrier”, Current Genetics, vol. 16, pp. 339-346, 1989.
Jones, “Tackling the Protease Problem inSaccharomyces cerevisiae”, Methods in Enzymology, vol. 194, 1991.
Barr Philip J.
Gibson Helen L.
Arriva Pharmaceuticals, Inc.
Goodwin & Procter LLP
Guzo David
Joike Michele K
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