System for detection of nitrosylated proteins

Details System for detection of nitrosylated proteins System for detection of nitrosylated proteins

2002-12-12

2008-09-02

Wallenhorst, Maureen M. (Department: 1797)

Chemistry: analytical and immunological testing

Nitrogen containing

Oxides of nitrogen

C436S063000, C436S086000, C436S164000, C436S172000, C436S106000, C422S067000, C422S082050, C422S082080

Reexamination Certificate

active

07419834

ABSTRACT:
The present invention describes a novel, simplified method for detecting and monitoring whether the presence of nitrosylated proteins, such as S-nitrosoproteins, in a biological sample using fluorescence detection. The present invention further describes a method which can both quantify and identify the nature of nitrosylated proteins, which method is useful for monitoring both normal and disease states, in the development and screening of potential therapeutic drug species.

REFERENCES:
patent: 5459076 (1995-10-01), Stamler et al.
patent: 5891735 (1999-04-01), Stamler et al.
Kojima et al. “Detection and Imaging of Nitric Oxide with Novel Fluorescent Indicators: Diaminofluoresceins” Analytical Chemistry, vol. 70, No. 13, Jul. 1, 1998.
Beckman et al., “Methods in Nitric Oxide Research,” Feelisch and Stainler, Wiley, Chichester, U.K. (1996).
Fang et al., “Reductive Assays for S-nitrosothiols: Implications for measurements in biological systems,” Bioche. Biophys. Res. Commun. 252:535-40 (1998).
Mannick et al., “Fas-induced capsase denitrosylation,” Science 284:65 (1999).
Wang et al., “Inhibition of papain by S-nitrosothiols. Formation of mixed disulfides,” J. Biol. Chem. 275(27):20467-73 (2000).
Itoh et al., “Determination and Bioimaging Method for Nitric Oxide in Biological Specimens by Diaminofluorescein Fluorometry,” Analytical Biochemistry, 287:203-209 (2000).
King et al., “Assessment of S-nitrosothiols on diaminofluorescein gels.” Analytical Biochemistry, 346:69-76 (2005).
Kojima et al., “Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia,” Journal of Neurochemistry, 76:1404-1410 (2001).

Affiliated with

Also associated with

Rating

System for detection of nitrosylated proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with System for detection of nitrosylated proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for detection of nitrosylated proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3967606