Method for detecting post-translation modifications of peptides

Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Amino acid or sequencing procedure

Reexamination Certificate

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C436S173000, C436S174000, C436S178000, C530S333000, C530S402000, C250S281000, C250S282000

Reexamination Certificate

active

07341874

ABSTRACT:
This invention provides for novel methods of identifying covalent modifications of an amino acid residue in a polypeptide chain by detecting mass differences.

REFERENCES:
patent: 5008372 (1991-04-01), Wellner
patent: 5045694 (1991-09-01), Beavis et al.
patent: 5118937 (1992-06-01), Hillenkamp et al.
patent: 5144006 (1992-09-01), Tam
patent: 5281538 (1994-01-01), Cottrell et al.
patent: 5288644 (1994-02-01), Beavis et al.
patent: 5328840 (1994-07-01), Coller
patent: 5432260 (1995-07-01), Stahl
patent: 6271037 (2001-08-01), Chait et al.
patent: 6750061 (2004-06-01), Chait et al.
patent: 529 604 (1993-03-01), None
patent: WO 94/06017 (1994-03-01), None
Chait BT, Wang R, Beavis RC, Kent SBH, Protein Ladder Sequencing, Science, 1993, 262: 89-92.
Hunt DF, Buko AM, Ballard JM, Shabanowitz J, Giordani AB, Sequence Analysis of Polypeptide by Collision Activated Dissociation on a Triple Quadrupole Mass Spectrometer, Biomedical Mass Spectrometry, 1981, 8(9): 397-408.
Qin J, Steenvoorden RJJM, Chait BT, A Practical Ion Trap Mass Spectrometer for the ANalysis of Peptides by Martix-Assisted Laser Desorption/Ionization, Analytical Chemistry, 1996, 68(10): 1784-1791.
Beavis RC, Chait BT, Rapic, Sensitive Analysis of Protein Mixtures by Mass Spectrometry, Proceedings of the National Academy of Sciences, U.S.A, 1990, 87: 6873-6877.
Cooks RG, Kaiser RE, Jr., Quadrupole Ion Trap Mass Spectrometry, Acc. Chem. Res., 1990, 23: 213-219.
Merrifield RB, Solid Phase Synthesis. I. The Synthesis of a Tetrapeptide, Journal of American Chemical Society, 1963, 85(14): 2149-2154.
Smith, L.A. et al., “Following Enzyme Catalysis in Real-Time Inside a Fast Atom Bombardment Mass Spectrometer”Biomed. Mass Spectrom10:98 (1983).
Tarr, G.E. “Improved Manual Sequencing Methods”Methods Enzymology47:355 (1977).
Kent, S.H. “Chemical Synthesis Of Peptides And Proteins”Annual Rev. Biochem. 57:957-984.
Tsugita et al “C-terminal sequencing of protein: A novel partial acid hydrolysis and analysis by mass spectrometry”Eur. J. Biochem. 206:691-696.
Chait et al. “A New Approach For Sequencing Peptides and Proteins”The 40th ASMS Conference, Jun. 5, 1992 pp. 1939-1940 (1992).
Beavis et al. “α-Cyano-4-hydroxycinnamic Acid as a Matrix for Matrix-assisted Laser Desorption Mass Spectrometry”Organic Mass Spectrometry27:156-158 (1992).
Aebersold et al. “Design, synthesis, and characterization of a protein sequencing reagent yielding amino acid derivatives with enhanced detectability by mass spectrometry”Protein Science1:494-503 (1992).
Schnolzer et al. “In situ neutralization in Boc-chemistry solid phase peptide synthesis”Int. J. Peptide Protein Res. 40:180-193 (1992).
Chait et al. “Weighing Naked Proteins: Practical, High-Accuracy Mass Measurement of Peptides and Proteins”Science257:1885-1894. (1992).
Hillenkamp et al. “Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Biopolymers”Anal. Chem. 63:1193-1202 (1991).
Beavis et al. “High-Accuracy Molecular Mass Determination of Proteins Using Matrix-Assisted Laser Desorption Mass Spectrometry”Anal. Chem. 62:1836-1840 (1990).
Beavis et al. “Factors Affecting the Ultraviolet Laser Desorption of Proteins”Rap. Comm. Mass Spec. 3:233 (1989).
Beavis et al. “Matrix Assisted UV Laser Desorption Of Biologically Interesting Molecules”The 37th ASMS Conference, Miami Beach, FL, pp. 1186-1187 (May 21-26, 1989).
Noren et al, “A General Method for Site-Specific Incorporation of Unnatural Amino Acids into Proteins”Science244:182-188 (1989).
Karas et al “Matrix-Assisted Ultraviolet Laser Desorption of Non-Volatile Compounds”International Journal Of Mass Spectrometry and Ion Processes, 78:53-68 (1987).
Chait et al., “Mass Spectrometric Characterization Of Microscale Enzyme Catalyzed Reactions Of Surface-Bound Peptides And Proteins”Methods In Protein Sequence Analysis edK.A. Walsh,The Humana Presspp. 483-492 (1986).
Karas et al. “Influence of the Wavelength in High-Irradiance Ultraviolet Laser Desorption Mass Spectrometry of Organic Molecules”Anal. Chem., 57:2935-2939 (1985).
Self, R. et al., “The Combined Use of Enzymatic Hydrolysis and Fast Atom Bombardment Mass Spectrometry for Peptide Sequencing”Biomedical Mass Spectrometry10:78-82 (1983).
Watson, J.D. et al. “Restriction Fragments Lead to Powerful New Methods for Sequencing DNA”Recombinant DNA—A Short Course, W.H. Freeman and Co., New York. pp. 61-63 (1983).
Tsugita et al “Exopeptidase Digestion In Combination With Field Desorption Mass Spectrometry For Amino Acid Sequence Determination”FEBS137:19-24 (1982).
Stryer, L.Biochemistry, 2nd Ed., W.H. Freeman and Co., New York, p. 663 (1981).
Schroeder, W. “Degradation of Peptides by the Edman Method with Direct Identification of the Phenylthiohydantoin-Amino Acid”Methods in Enzymol. 25:298-313 (1972).
Richards, F. et al., “Mass Spectroscopy of Methylthiohydantoin Amino Acids: Identification, Quantitation, and the Analysis of Mixtures”Methods in Enzymol., 25:314-325 (1972).
Konigsberg, W. “Subtractive Edman Degradation”Methods in Enzymol. 25:326-332 (1972).

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