Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-05-13
2008-05-13
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C536S024300
Reexamination Certificate
active
11241990
ABSTRACT:
Recently two techniques using free solution electrophoresis to separate charged-uncharged polymer conjugates have proven successful: End Labeled Free Solution Electrophoresis (ELFSE) for DNA sequencing, and Free Solution Conjugate Electrophoresis (FSCE) for molar mass profiling of uncharged polymers. Previous attempts have been made to analyze experimental data generated by these new techniques for the electrophoresis of molecules with varying charge distributions. However, the importance of the ends of the polymers in determining the polymer's overall mobility was neglected in previous work. Through a careful investigation and a reanalysis of the experimental data, it is determined here that this “end effect” critically impacts the behavior of polymers and charged-uncharged polymer conjugates during electrophoresis. In this way, the invention provides for methods that exploit this “end effect” for the separation of polymeric molecules on the basis of size, including for example DNA separation and sequencing techniques.
REFERENCES:
patent: 2003/0088056 (2003-05-01), Barron
Author(s): Ren, H., Karger, A.E., Oaks, F., Menchen, S., Slater, G.W., Drouin, G., Title: Separating DNA sequencing fragments without a sieving matrix Source: Electrophoresis Year; 1999 Chapter 20 pp. 2501-2509.
Author(s): Völkel, A.R., Noolandi, J. Title: Mobilities of Labeled and Unlabeled Single-Stranded DNA in Free Solution Electrophoresis Source: Macromolecules Year: 1995 Chapter 28 pp. 8182-8189.
Author(s): Mayer, P., Slater, G.W., Drouin, G. Title: Theory of DNA Sequencing Using Free-Solution Electrophoresis of Protein-DNA Complexes Source: Anal. Chem. Year: 1994 Chapter 66 pp. 1777-1780.
Author(s): Heller, C., Slater, G.W., Mayer, P., Dovichi, N., Pinto, D., Viovy, J.-L., Drouin, G., Title: Free-solution electrophoresis of DNA Source: J. Chrom. A. Year: 1998 Chapter 806 pp. 113-121.
Author(s): Desruisseaux, C., Long, D., Drouin, G., Slater, G.W. Title: Electrophoresis of Composite Molecular Objects. 1. Relation between Friction, Charge, and Ionic Strength in Free Solution Source: Macromolecules Year: 2001 Chapter 34 pp. 44-52.
Author(s); Desruisseaux, C., Drouin, G., Slater, G.W. Title; Electrophoresis of Composite Molecular Objects. 2. Competition between sieving and Frictional Effects in Polymer Solutions Source; Macromolecules Year: 2001 Chapter 34 pp. 5280-5286.
Author(s): Viovy, J.L. Title: Electrophoresis of DNA and other polyelectrolytes: Physical mechanisms Source: Rev. Mod. Phys. Year: 2000 Chapter 72 pp. 813-872.
Author(s): Olivera, B.M., Baine, P., Davidson, N. Title: Electrophoresis of the Nucleic Acids Source: Biopolymers Year: 1964 Chapter 2 pp. 245-257.
Author(s): Stellwagen, N.C., Gelfi, C., Righetti, P.G. Title: The Free Solution Mobility of DNA Source: Biopolymers Year: 1997 Chapter 42 pp. 687-703.
Author(s): Stellwagen, N.C., Stellwagen, E. Title: The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl Source: Electrophoresis Year: 2002 Chapter 23 pp. 1935-1941.
Author(s): Vreeland, W.N., Desruisseaux, C., Karger, A.E., Drouin, G., Slater, G.W., Barron, A.E. Title: Molar Mass Profiling of Synthetic Polymers by Free-Solution Capillary Electrophoresis of DNA-Polymer Conjugates Source: Anal. Chem. Year: 2001 Chapter 73 pp. 1795-1803.
Author(s): Vreeland, W.N., Slater, G.W., Barron, A.E. Title: Profiling Solid-Phase Synthesis Products by Free-Solution Conjugate Capillary Electrophoresis Source: Bioconj. Chem. Year: 2002 Chapter 13 pp. 663-670.
Author(s): McCormick, L.C., Slater, G.W., Karger, A.E., Vreeland, W.N., Barron, A.E., Desruisseaux, C., Drouin, G. Title: Capillary electrophoretic separation of uncharged polymers using polyelectrolyte engines Source: Electrophoresis Year: 2001 Chapter 924 pp. 43-52.
Author(s): Long, D. Dobrynin, A.V., Rubinstein, M., Ajdari, A., Title: Electrophoresis of polyampholytes Source: J. Chem. Phys. Year: 1998 Chapter 108 pp. 1234-1244.
Author(s): Won, J.-I., Meagher, R.J., Barron, A.E. Title: Characterization of Glutamine Deamidation in a Long, Repetitive Protein Polymer via Bioconjugate Capillary Electrophoresis Source: Biomacromolecules Year: 2004 Chapter 5 pp. 618-627.
Author(s): Noolandi, J. Title: A New Concept for Sequencing DNA by Capillary Electrophoresis Source: Electrophoresis Year: 1992 Chapter 13 pp. 394-395.
Author(s): Meagher, R.J. Won, J.I., McCormick, L.C. Nedelcu, S., Bertrand, M. M. Title: End-labeled free-solution electrophoresis of DNA Source: Electrophoresis Year: 2005 Chapter 26 pp. 331-350.
Author(s): Bullock, J. Source: J. Chrom. Year: 1993 Chapter 645 pp. 169-177.
Author(s): Zuckermann, R. N., Kerr, J. M., Kent, S. B. H., Moos, W. H.. Title: Efficient Method For the Preparation of Peptoids [Oligo(N-Substituted Glycines)] By Submonomer Solid-Phase Synthesis Source: J. Am. Chem. Soc. Year: 1992 Chapter 114 pp. 10646-10647.
Author(s): Vreeland, W. N., Barron, A. E. Title: Free-solution capillary electrophoresis of polypeptoid- oligonucleotide conjugates Source: Abstracts of Papers of the American Chemical Society Year: 2000 Chapter 219 pp. 555-556.
Author(s): Vreeland, W.N., Meagher, R. J., Barron, A. E. Title: Multiplexed, High-Throughput Genotyping by Single-Base Extension and End-Labeled Free-Solution Electrophoresis Source: Anal. Chem. Year: 2002 Chapter 74 pp. 4328-4333.
Author(s): Haynes, R. D., Meagher, R.J., Won, J. I., Bogdan, F. M. Barron, A. E. Title: Comb-like, monodisperse polypeptoid drag-tags for DNA separation by End-Labeled Free-Solution Electrophoresis (ELFSE) Source: Bioconjugate Chem. Year: 2005 Chapter 16 pp. 929-938.
Author(s): Won, J. I., Barron, A. E. Title: A new cloning method for preparation of long repetitive polypeptides without sequence requirement Source: Macromolecules Year: 2002 Chapter 35 pp. 8281-8287.
Author(s): Won, J. I. Meagher, R. J., Barron, A. E. Title: Protein polymer drag-tags for DNA separations by end-labeled free-solution electrophoresis Source: Electrophoresis Year: 2005 Chapter 26 pp. 2138-2148.
Author(s): Doherty, E. A. S., Berglund, K. D., Buchholz, B. A., Kourkine, I. V., Przybycien, T.M. Title: Critical factors for high-performance physically adsorbed (dynamic) polymeric wall coatings for capillary electrophoresis of DNA Source: Electrophoresis Year: 2002 Chapter 23 pp. 2766-2776.
Author(s): McCormick, L. C., Slater, G. W., Karger, A. E., Vreeland, W. N., Barron, A. E. Title: Capillary electrophoretic separation of uncharged polymers using polyelectrolyte engines—Theoretical model Source: J. Chromatogr. A Year: 2001 Chapter 924 pp. 43-52.
Author(s): Haynes, R. D., Meagher, R. J., Coyne, J. Barron, A. E. Title: Comb-like poly-N-substituted glycines as end-labeled free-solution electrophoresis (ELFSE) drag-tags for DNA sequencing Source: Abstracts of the Papers of the American Chemical Society Year: 2006 Chapter 231 pp. 131-ORGN, 26.
Author(s): Meagher, R. J., McCormick, L. C., Haynes, R. D., Won, J. I., Lin, J. S., Slater, G. W., Barron, A. E. Title: Free-solution electrophoresis of DNA modified with drag-tags at both ends Source: Electrophoresis Year 2006 Chapter 27 pp. 1702-1712.
Author(s): McCormick, L. C., Slater, G. W. Title: A theroetical study of the possible use of electroosmotic flow to extend the read length of DNA sequencing by end-labeled free solution electrophoresis Source: Electrophoresis Year: 2006 Chapter 27 pp. 1693-1701.
Author(s): Nedelcu, S., Slater, G. W. Title: Branched polymeric labels used as drag-tags in free-solution electrophoresis of ssDNA Source: Electrophoresis Year: 2005 Chapter 26 pp. 4003-4015.
Author(s): McCormick, L. C., Slater, G. W. Title: The molecular end effect and its critical impact on the behavior of charged-uncharged polymer conjugates during free-solution electrophoresis Source: Electrophoresis Year: 2005 Chapter 26 pp. 1659-1667.
Author(s): Völkel, A. R., Noolandi, J. Title: Mobilities of Labeled and Unlabeled Single-Stranded DNA in Free Solution Electrophoresis Source: Macromolecules Year: 1995 Chapter 28 pp. 8182-8189.
Au
Barron Annelise E
McCormick Laurette C.
Meagher Robert J.
Slater Gary W.
Northwestern University
Osler Hoskin & Harcourt LLP
University of Ottawa
Whisenant Ethan
LandOfFree
Methods for separation of polymeric compounds does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for separation of polymeric compounds, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for separation of polymeric compounds will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3957444