Nucleic acid adsorbent; methods for adsorbing, removing,...

Details Nucleic acid adsorbent; methods for adsorbing, removing,... Nucleic acid adsorbent; methods for adsorbing, removing,...

2008-04-15

2008-04-15

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S007100, C435S007200, C536S023100

Reexamination Certificate

active

11214773

ABSTRACT:
A nucleic acid adsorbent in which a polymer having a constitutional unit represented by the following formula (I) is immobilized on a water-insoluble carrier is used as a nucleic acid adsorbent capable of selectively and efficiently adsorbing nucleic acids from complex mixtures, such as cell extract,wherein R1represents hydrogen, fluoride, or methyl; R2and R3each independently represent alkyl of 1 to 6 carbon atoms; A is —NH— or —O—; and B represents alkylene of 2 to 18 carbon atoms. Nucleic acids are recovered from cell extract by allowing them to be adsorbed to the nucleic acid adsorbent by mixing this nucleic acid adsorbent with cell extract, removing the nucleic acids from the cell extract by separating the adsorbent therefrom, and dissociating the nucleic acids from the nucleic acid adsorbent by treating the separated nucleic acid adsorbent with alkali.

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