Method for enriching adherent monocyte populations

Details Method for enriching adherent monocyte populations Method for enriching adherent monocyte populations

2008-07-01

2008-07-01

Saucier, Sandra (Department: 1651)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C424S534000

Reexamination Certificate

active

10530748

ABSTRACT:
The present invention provides methods and apparatus for the rapid and efficient collection and purification of activated monocytes. The methods and apparatus of the present invention provide means for effecting the collection and purification in an aseptic environment. The method involves filtering a blood component mixture through a monocyte-adhering filter; washing the blood component mixture; backflushing the filter with a physiological solution; and backflushing the filter with Dextran-40/serum albumin.

REFERENCES:
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patent: 6129853 (2000-10-01), Sasayama et al.
patent: 7-80062 (1995-03-01), None
Ebner et al., “Generation of large numbers of human dendritic cells from whole blood passaged through leukocyte removal filters: an alternative to standard buffy coats”, J. Immunological Methods 252 : 93-104 (2001).

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