Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-07-08
2008-07-08
Kim, Young J (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S006120
Reexamination Certificate
active
09816763
ABSTRACT:
The present invention is related to a screening, detection and/or quantification method of one or more transcriptional factor(s) (1) possibly present in a biological sample, said method comprising the steps of:possibly extracting and isolating said transcriptional factor (1) from said biological sample,putting into contact the transcriptional factor (1) with a double-stranded DNA sequence (2) bound to an insoluble solid support (3), anddetecting and/or quantifying said fixed transcriptional factor (1),said double-stranded DNA sequence having a specific sequence able to be fixed by the transcriptional factor (1) and being preferably located at a distance of at least about 6.8 nm from the surface of the solid support (3), and said double-stranded DNA sequence being bound to the surface of the insoluble solid support (3) at a concentration of at least 0.01 pmole/cm2of solid support surface (3).The present invention is also related to the kit comprising means and media for performing said method.
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Art Muriel
Remacle Jose
Renard Patricia
Eppendorf Array Technologies S.A.
Kim Young J
Knobbe Martens Olson & Bear LLP
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