Method for determining the length of a nucleic acid molecule

Details Method for determining the length of a nucleic acid molecule Method for determining the length of a nucleic acid molecule

2007-02-13

2007-02-13

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C536S023100, C422S082080

Reexamination Certificate

active

10220330

ABSTRACT:
A method of determining the length of a particular region within a nucleic acid, the method comprising a) subjecting a sample of the nucleic acid to a plurality of amplification reactions in which the region is amplified, wherein the time of the extension phase in each of the reactions is varied; b) monitoring the progress of the amplification reactions; c) determining the minimum time during which extension phase of the amplification is completed within each reaction mixture and relating that to the length of the sequence undergoing extension. The method, combined with melting point analysis, will allow percentage GC content of a sequence to be determined. Length analysis of this type can be used in diagnosis or analysis as well as in recombinant DNA technology to check for the presence of concatamers, and in taxonomic classification or forensics. Apparatus for use in the method is also described and claimed.

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