Sustained totipotent culture of selected monocot genera

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se

Reexamination Certificate

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C435S430000, C435S420000

Reexamination Certificate

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10982254

ABSTRACT:
A method is provided for generating sustained totipotent tissue cultures of a plant of the Class Monocotyledonae, and for micropropagating such plant in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons.

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