Homogenous assay for enzymatic activity

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S015000, C435S021000, C435S023000

Reexamination Certificate

active

10517758

ABSTRACT:
An assay is disclosed for measuring activity of enzymes, such as kinases, phosphatases, and proteases. Measurements of enzymatic activity are accomplished in a homogenous assay format utilizing a fluorescence quenching technique employing paramagnetic metal ions.

REFERENCES:
patent: 5034189 (1991-07-01), Cox et al.
patent: 6472141 (2002-10-01), Nikiforov
patent: 6689565 (2004-02-01), Nikiforov
patent: 6699655 (2004-03-01), Nikiforov
patent: WO-00/72016 (2000-11-01), None
patent: WO-01/07638 (2001-02-01), None
International Search Report (3 pages), 2003.

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