Recombinant poxvirus comprising at least two compox ATI...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

Reexamination Certificate

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C424S186100, C435S005000, C435S006120, C435S091100, C435S091330

Reexamination Certificate

active

10535371

ABSTRACT:
Recombinant poxvirus are disclosed comprising in the viral genome at least two expression cassettes, each comprising a cowpox ATI promoter according to SEQ ID NO:1, a polynucleotide sequence in which not more than 6 nucleotides are substituted, deleted, and/or inserted into SEQ ID NO:1 and still active as an ATI promoter, or a polynucleotide comprising at least 10 nucleotides including nucleotides 22 to 29 of SEQ ID NO: 1 and still active as an ATI promoter and a coding sequence, wherein the expression of the coding sequence is regulated by said promoter or said polynucleotides. The recombinant poxviruses are useful as pharmaceutically active ingredients in the preparation of vaccines.

REFERENCES:
patent: 5443964 (1995-08-01), Pickup et al.
patent: WO 97/02355 (1997-01-01), None
patent: WO 02/18585 (2002-03-01), None
Blanchard et al. Modified vaccinia virus Ankara undergoes limited replication in human cells and lacks several immunomodulatory proteins: implications for use as a human vaccine, Journal of General Virology, 1998, vol. 79, pp. 1159-1167.
A Vaccinia Virus Transfer Vector . . . by M. Howley et al. (Gen2, 172 (1996).
Conserved Taaat Motif in Vaccinia Virus . . . by M. Hänggi et al. (FMBO Journal, 1986).
High-Level Expression of Amsacta Moorei . . . by Y. Ki et al. (Journal of Gen. Virology 1998).

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