Purified plant expansin proteins and DNA encoding same

Details Purified plant expansin proteins and DNA encoding same Purified plant expansin proteins and DNA encoding same

2007-06-05

2007-06-05

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C536S023200, C536S023500, C536S023600, C435S320100, C435S069100, C435S252300

Reexamination Certificate

active

10670009

ABSTRACT:
This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified inArabidopsisand an additional two in rice.

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