Recombinant P. falciparum merozoite protein-1 42 vaccine

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C530S300000, C530S350000, C435S069100, C435S069700, C435S173300, C435S320100, C424S272100, C424S268100, C424S265100, C424S199100, C424S191100, C424S185100

Reexamination Certificate

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10404667

ABSTRACT:
In this application is described the expression and purification of a recombinantPlasmodium falciparum(FVO) MSP-142. The method of the present invention produces a highly purified protein that retains folding and disulfide bridging of the native molecule. The recombinant MSP-142is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

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patent: WO-01/34188 (2001-05-01), None
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Kumar, S. et al., 2002. A DNA vaccine encoding the 42 kDa C-terminus of merozoite surface protein 1 ofPlasmodium falciparuminduces antibody, interferon-γ and cytotoxic T cell responses in rhesus monkeys: immuno-stimulatory effects of granulocyte macrophage-colony stimulating factor. Immunology Letters 81, 13-24.
Angov, E. et al., 2003. Development and pre-clinical analysis of aPlasmodium faciparummerozoite surface protein-142malaria vaccine. Molecular & Biochemical Parasitology 128, 195-204.
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Pang et al., “In Vivo Expression and Immunological Studies of the 42-Kilodalton Carboxyl-Terminal Processing Fragment ofPlasmodium falciparumMerozoite Surface Protein 1 in the Baculovirus-Silkworm System”, Infection and Immunity, Jun. 2002, pp. 2772-2779, vol. 70, No. 6.
Qian et al., “Inducible Expression of MSP1 Gene ofPlasmodium falciparumby a Tetracycline-Controlled Promoter”, Chinese Journal of Parasitology & Parasitic Diseases, 2000, pp. 193-196, vol. 18, No. 4. (with English Abstract).
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