Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2007-02-20
2007-02-20
Qian, Celine (Department: 1636)
Chemistry: molecular biology and microbiology
Vector, per se
Reexamination Certificate
active
10117825
ABSTRACT:
Methods are provided for producing an expression vector. In the subject methods, donor and acceptor vectors are combined in the presence of a recombinase to produce an expression vector that includes a first and second recombinase recognition site oriented in the same direction, wherein the first and second recombination sites are able to recombine with each other. In the subject methods, one of the donor and acceptor vectors includes a single recombinase recognition site while the other includes two recombinase recognition sites. Also provided are compositions for use in practicing the subject methods, including the donor and acceptor vectors themselves, as well as systems and kits that include the same. The subject invention finds use in a variety of different applications, including the transfer or cloning of a nucleic acid of interest from a first vector into one or more expression vectors, etc.
REFERENCES:
patent: 4959317 (1990-09-01), Sauer
patent: 5378618 (1995-01-01), Sternberg et al.
patent: 5527695 (1996-06-01), Hodges et al.
patent: 5851808 (1998-12-01), Elledge et al.
patent: 5888732 (1999-03-01), Hartley et al.
patent: 5962255 (1999-10-01), Griffiths et al.
patent: 6010884 (2000-01-01), Griffiths et al.
patent: 0 559 725 (1991-11-01), None
patent: WO 96/30498 (1996-03-01), None
patent: WO 96/40724 (1996-12-01), None
patent: WO 00/05355 (1998-07-01), None
patent: WO 00/12687 (2000-03-01), None
Webster's II New Riverside University Dictionary, unnamed authors, published by The Riverside Publishing Company, 1994, p. 1086.
Seibler et al Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay. Biochemistry (1997) vol. 36, pp. 1740-1747.
Baubonis et al.(1993), “Genomic Targeting with Purified Cre Recombinase,”Nucleic Acids Research, vol. 21(9): 2025-2029.
Bayley et al. (1992), “Exchange of Gene Activity in Transgenic Plants Catalyzed by the Cre-lox Site-Specific Recombination System,”Plant Molecular Biology, vol. 18: 353-361.
Bevan Michael (1984), “Binary Agrobacterium Vectors for Plant Transformation,”Nucleic Acids Research, vol. 12, No. 22.
Fukushige et al. (1992), “Genomic Targeting with a Positive-Selection Lox Integration Vector Allows Highly Reproducible Gene Expression in Mammalian Cells,”Proc. Natl. Acad. Sci. USA, vol. 89 pp. 7905-7909.
Liu et al. (1998), “The Univector Plasmid-Fusion System, A Method for Rapid Construction of Recombinant DNA without Restriction Enzymes,”Current Biology, vol. 8: 1300-1309.
Pósfai et al. (1994), “In Vivo Excision and Amplification of Large Segments of theEscherichia coloGenome,”Nucleic Acids Research, vol. 22 (12): 2392-2398.
Qin et al. (1994), “Cre Recombinase-Mediated Site-Specific Recombination Between Plant Chromosomes,”Proc. Natl. Acad. Sci. USA, vol. 81: 1706-1710.
Sauer et al. (1989), “Cre-Stimulated Recombination atloxP-Containing DNA Sequences Placed into the Mammalian Genome,”Nucleic Acids Research, vol. 17 (1): 147-161.
Sauer et al. (1987), “Site-Specific Insertion of DNA into a Pseudorabies Virus Vector,”Proc. Natl. Acad. Sci. USA, vol. 84: 9108-9112.
Sauer et al. (1992), “Construction of Isogenic Cell Lines Expressing Human and Rat Angiotensin II AT1 Receptors by Cre-Mediated Site Specific Recombination,”METHODS: A Companion to Methods in Enzymology, vol. 4: 143-149.
Sauer et al. (1990), “Targeted Insertion of Exogenous DNA into the Eukaryotic Genome by the Cre Recombinase,”The New Biologist, vol. 2 (5): 441-449.
Ronald Hoess et al. “Formation of small circular DNA molecules via an vitro site-specific recombination system”Gene4 (1985) 325-329.
Cydne L. Holt et al. “A novel phage λreplacement Cre-lox vector that has automatic subcloning capabilities”Gene133 (1993) 95-97.
Bozicevic Field & Francis LLP
Clontech Laboratories, Inc.
Field Bret E.
Garvey Tara L.
Qian Celine
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