Nucleic acid detection method

Details Nucleic acid detection method Nucleic acid detection method

2007-10-16

2007-10-16

Lu, Frank (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091100, C536S023100, C536S024300, C536S024330, C536S025300

Reexamination Certificate

active

10388329

ABSTRACT:
Disclosed are methods for detecting a target nucleic acid molecule having a known sequence among a plurality of nucleic acid molecules. The method includes hybridizing two oligonucleotides to adjacent nucleotide sequences on the target nucleic acid molecule, ligating the two oligonucleotides together, and extending a primer that hybrizes to the 3′ end of the oligonucleotide that hybrized to the 5′ nucleotide sequence on the target nucleic acid molecule. If the target nucleic acid molecule is present (and thereby allowing ligation of the two oligonucleotides), extension of the primer dislodges a detection probe hybridized to the 5′ end of the oligonucleotide that hybridized to the 3′ nucleotide sequence on the target nucleic acid molecule.Also disclosed are kits for performing the methods of the invention.

REFERENCES:
patent: 5679524 (1997-10-01), Nikiforov et al.
patent: 5736330 (1998-04-01), Fulton
patent: 5770365 (1998-06-01), Lane et al.
patent: 5800994 (1998-09-01), Martinelli et al.
patent: 5830711 (1998-11-01), Barany et al.
patent: 5837860 (1998-11-01), Anderson et al.
patent: 5863722 (1999-01-01), Brenner
patent: 5866337 (1999-02-01), Schon
patent: 5914229 (1999-06-01), Loewy
patent: 6013431 (2000-01-01), Söderlund et al.
patent: 6020137 (2000-02-01), Lapidus et al.
patent: 6027889 (2000-02-01), Barany et al.
patent: 6030782 (2000-02-01), Anderson et al.
patent: 6057107 (2000-05-01), Fulton
patent: 6140489 (2000-10-01), Brenner
patent: 6150516 (2000-11-01), Brenner et al.
patent: 6172214 (2001-01-01), Brenner
patent: 6172218 (2001-01-01), Brenner
patent: 6184000 (2001-02-01), Jones et al.
patent: 2295011 (1996-05-01), None
patent: 2295228 (1996-05-01), None
patent: WO96/15271 (1996-05-01), None
patent: WO96/31622 (1996-10-01), None
patent: WO99/22030 (1999-05-01), None
patent: WO99/67414 (1999-12-01), None
patent: WO99/67641 (1999-12-01), None
patent: WO00/24939 (2000-05-01), None
patent: WO00/39345 (2000-07-01), None
patent: WO00/39587 (2000-07-01), None
patent: WO00/40755 (2000-07-01), None
patent: WO00/47996 (2000-08-01), None
patent: WO00/48000 (2000-08-01), None
patent: WO00/53806 (2000-09-01), None
patent: WO00/67894 (2000-11-01), None
patent: WO01/11333 (2001-02-01), None
patent: WO01/13120 (2001-02-01), None
patent: WO01/18524 (2001-03-01), None
patent: WO 01/61033 (2001-08-01), None
patent: WO 01/92579 (2001-12-01), None
patent: WO 03/002762 (2003-01-01), None
patent: WO 03/052140 (2003-06-01), None
European Search Report corresponding to European Application No. 0376551.1 dated Oct. 6, 2005.
Shi Michael M., “EnablinG Large-Scale Pharmacogenetic Studies by High-Throughput Mutation Detection and Genotyping Technologies”,Clinical Chemistry, 47 (2): 164-172, 2001.
Iannone et al., “Multiplexed Single Nucleotide Polymorphism Genotyping by Oligonucleotide Ligation and Flow Cytometry”,Cytometry, 39; 131-140, 2000.
Notification of Transmittal of International Preliminary Examination Report corresponding to PCT Application Serial No. PCT/US03/07818 dated Oct. 17, 2006.
Communication pursuant to Article 96(2) EPC corresponding to EP Patent Application No. 03716551.1 dated Sep. 20, 2006.

Affiliated with

Also associated with

Rating

Nucleic acid detection method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid detection method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid detection method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3854548