Discrimination method of target base in DNA, and allele...

Details Discrimination method of target base in DNA, and allele... Discrimination method of target base in DNA, and allele...

2007-11-20

2007-11-20

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100

Reexamination Certificate

active

11488635

ABSTRACT:
An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-TA-3′, 5′-TT-3′ or 5′-CT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

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