Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Reexamination Certificate
2007-08-21
2007-08-21
McKenzie, Thomas (Department: 1625)
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
C549S511000
Reexamination Certificate
active
11028487
ABSTRACT:
This invention relates to methods for purification of paclitaxel from a paclitaxel-containing material. The method comprises the following steps: (a) extracting a paclitaxel-containing material with an organic solvent to obtain an extract, and concentrating the extract; (b) adding the concentrate with an organic solvent which is not mixed with water to separate an organic solvent phase and then concentrating; (c) subjecting the concentrate to normal phase chromatography to obtain an eluate; (d) dissolving the eluate in an acetone or dichloromethane followed by adding pentane or hexane to form a precipitate; and (e) subjecting the precipitate to high performance liquid chromatography. According to the method of the present invention, paclitaxel of over 99.5% purity can be easily obtained from aTaxusgenus plant with a high yield.
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Zhang, Z., “Separation and Purification of Taxol Using Normal and Reversed Phase Chromatography in Tandem”,Sheng Wu Gong Cheng Xue Bao Chinese Journal of Biotechnology, vol. 16, No. 1, pp. 69-73 (Jan. 2001) with English-language abstract.
Kim, J., et al. “Development of High Performance Liquid Chromatography for Paclitaxel Purification from Plant Cell Cultures”,Journal of Microbiology and Biotechnology, vol. 11, No. 2, pp. 204-210 (Apr. 2001).
Cho Jin-Suk
Choi Ho-Joon
Kim Moon-Suk
Pyo Sang-Hyun
Song Bong-Kyu
Ladas & Parry LLP
McKenzie Thomas
Rahmani Niloofar
Samyang Genex Corporation
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