Materials and methods for binding nucleic acids to surfaces

Details Materials and methods for binding nucleic acids to surfaces Materials and methods for binding nucleic acids to surfaces

2007-07-24

2007-07-24

Jiang, S. Anna (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S027120, C536S027210, C536S027800, C536S026230, C544S267000

Reexamination Certificate

active

11057440

ABSTRACT:
Surfaces containing high purity silica (silicon dioxide) exhibit high loading potential for nucleic acids.Formulations containing nucleic acids and materials which mask the electrostatic interactions between the nucleic acids and surfaces are disclosed. By masking the phosphate charges of the nucleic acids, undesired interactions may be minimized or eliminated, thereby allowing the covalent bonding of the nucleic acids to the surface to proceed. The use of such formulations additionally minimizes nonspecific binding of the nucleic acids to the surface. Examples of materials to be included in such formulations include cations, xanthines, hexoses, purines, arginine, lysine, polyarginine, polylysine, and quaternary ammonium salts.

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