Protein production

Details Protein production Protein production

2007-02-27

2007-02-27

Wax, Robert A. (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Reexamination Certificate

active

10528406

ABSTRACT:
Proteins may be made by genetically engineered microorganisms, the protein being stored in the form of inclusion bodies (IB). The proteins in the inclusion bodies are in an insoluble and inactive form. They may be dissolved using a solubilization reagent (18), and the resulting solution diluted so that the proteins refold into the active form. This refolding of the protein is enhanced by subjecting a solution or suspension of the protein to low intensity sound waves (25), at a low enough intensity that the protein is not denatured. The intensity may be between 10 and 100 mW/cm2.

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V. Nornstein et al, “Changes of Surface Tension of Human Serum Albumin Induced by Ultrasonic Cavitation and by pH-Lowering,” Studia Biophysica, vol. 125, 1988, No. 3, pp. 179-186.

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