Methods for enhancing the translation and expression of...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S069100, C435S252310, C435S252350, C435S252500, C536S024100, C536S023200

Reexamination Certificate

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10122882

ABSTRACT:
The increased use of nucleotide sequence data mining techniques has amplified the demand for efficient methods of producing recombinant proteins in prokaryotic cells. A strategy is provided for enhancing the synthesis of recombinant amino acid sequences by improving translation from expression cassettes in vitro before producing recombinant hosts.

REFERENCES:
patent: 5192669 (1993-03-01), Schoner et al.
patent: 5691169 (1997-11-01), Dalbøge et al.
patent: 6184356 (2001-02-01), Anderson et al.
patent: 3843894 (1988-12-01), None
patent: 0392605 (1990-10-01), None
Schoner et al, Enhanced Translational Efficiency with Two-Cistron Expression Systems, Methods in Enzymology, vol. 85, pp. 94-103.
Schoner et al., “Translation of a synthetic two-cistron mRNA inEscherichia coli”,Biochemistry 83:8506-8510, Nov. 1986.
Schoner et al., “Enhanced Translational Efficiency with Two-Cistron Expression System”,Methods in Enzymology 185:94-103, 1990.
Shine et al., “The 3'-Terminal Sequence ofEscherichia coli16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites”,Proc. Nat. Acad. Sci. 71(4):1342-1346. Apr. 1974.
Thomas et al., “Excherchia coliplasmid vectors containing synthetic translational initiation sequences and ribosome binding sites fused with thelacZgene”,Gene 19:211-219, 1982.
De Smit et al., “Secondary structure of the ribosome binding site determines translational efficiency: A quantitative analysis”,Proc. Natl. Acad. Sci. 87: 7668-7672, Oct. 1990.
Sprengart et al., “The downstream box: an efficient and independent translation initiation signal inEscherichia coli”,The EMBO Journal 15(3)665-674, 1996.
Etchegaray et al., “Translational Enhancement by an Element Downstream of the Initiation Codon inEschetichia coli”,The Journal of Biological Chemistry 274(15):10079-10085, Apr. 1999.
Ito et al., “Multiple control ofEscherichia colilysyl-tRNA synthetase expression involves a transcriptional repressor and a translational enhancer element”,Proc. Natl. Acad. Sci. 90:302-306, Jan. 1993.
Makoff et al., “The use of two-cistron constructions in improving the expression of a heterologous gene inE. coli”,Nucleic Acids Research 18(7):1711-1718, 1990.
Pedersen et al., “Removal of N-Terminal Polyhistidine Tags from Recombinant Proteins Using Engineered Aminopeptidases”,Protein Expression and Purification 15:389-400, 1999.
Pryor et al., “High-Level Expression of Soluble Protein inEscherichia coliUsing a His6-Tag and Maltose-Binding-Protein Double-Affinity Fusion System”,Protein Expression and Purification 10:309-319, 1997.
Unizyme Laboratories trade show brochure, 1999.
Unizyme Laboratories product insert, 1999.

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