Method for assaying DNA fragments in mixture

Details Method for assaying DNA fragments in mixture Method for assaying DNA fragments in mixture

2007-07-10

2007-07-10

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C536S023100, C536S024300

Reexamination Certificate

active

10634795

ABSTRACT:
The inventive method for assaying DNA fragments in mixture comprisesstep 1 of ligating different oligomers hybridizable to primers of the same melting temperature and the same length to individual groups of DNA fragments in a set of DNA fragments;step 2 of mixing together the groups of DNA fragments ligated with the oligomers;step 3 of simultaneous PCR of the groups of DNA fragments ligated with the oligomers in one receptacle by using the primers being complementary to the oligomers and corresponding to the individual groups; andstep 4 of detecting PCR amplified DNA fragments; characterized in that the method enables the comparison of plural samples under no influence of PCR reproducibility.

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