Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of culturing encapsulated cells
Reexamination Certificate
2007-07-03
2007-07-03
Campell, Bruce R. (Department: 1648)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of culturing encapsulated cells
C435S325000, C435S235100, C435S320100, C435S366000
Reexamination Certificate
active
10241024
ABSTRACT:
Methods for efficient production of recombinant AAV employ a host cell which comprising AAV rep and cap genes stably integrated within the cell's chromosomes, wherein the AAV rep and cap genes are each operatively linked to regulatory sequences capable of directing the expression of the rep and cap gene products upon infection of the cell with a helper virus, a helper gene, and a helper gene product. A method for producing recombinant adeno-associated virus (rAAV) involves infecting such a host cell with a helper virus, gene or gene product and infecting the infected host cell with a recombinant hybrid virus or plasmid vector containing adenovirus cis-elements necessary for replication and virion encapsidation, AAV sequences comprising the 5′ and 3′ ITRs of an AAV, and a selected gene operatively linked to regulatory sequences directing its expression, which is flanked by the above-mentioned AAV sequences.
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Gao Guangping
Wilson James M.
Campell Bruce R.
Howson & Howson LLP
Peng Bo
The Trustees of the University of Pennsylvania
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