Apparatus for nucleic acid quantification

Details Apparatus for nucleic acid quantification Apparatus for nucleic acid quantification

1983-02-04

1985-07-02

Sadowski, David

Liquid purification or separation

Plural distinct separators

Filters

2104332, 210489, 210492, 210927, 422101, B01D 3100

Patent

active

045266905

ABSTRACT:
A multiwell apparatus for the assay of microliter quantities of body fluids is provided which prevents fluid loss by lateral migration or gravity flow through a microporous membrane or ultrafilter. A sample is passed through a membrane to which an antibody has been bonded in order to harvest complementary viral antigens in the sample. The harvested viral antigen is converted to single strand DNA or RNA which then is deposited on a second membrane. The deposited single strand DNA or RNA is capable of reacting with labeled DNA or other suitable detection probe which can be detected and correlated with antigen concentration in the sample.

REFERENCES:
patent: Re30562 (1981-03-01), Park
patent: 3649464 (1972-03-01), Freeman
patent: 4154795 (1979-05-01), Thorne
patent: 4220535 (1980-09-01), Leonard
patent: 4245064 (1981-01-01), Drobnik et al.
patent: 4246339 (1981-01-01), Cole et al.
patent: 4276048 (1981-06-01), Leaback
patent: 4302204 (1981-11-01), Wahl et al.
patent: 4304865 (1981-12-01), O'Brien et al.
patent: 4338094 (1982-07-01), Elahi
Millipore Catalogue and Purchasing Guide, 1977, First Printing Dec. 1976, Cat. No. MC177/u, Millipore Corp., Bedford, MA 01730, p. 48.

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