Matrixes, arrays, systems and methods

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S610000

Reexamination Certificate

active

10198071

ABSTRACT:
The present invention relates to matrixes, arrays, systems and methods for analyzing biomolecules by their isoelectric point, optionally, in combination with a second dimension analysis. The assortment of matrixes, arrays and systems provided herein are useful for causing a biomolecule under the influence of an electrical field to accumulate into an IEF buffer that comprises a pH value that is the same as the isoelectric point of the biomolecule. The methods of this invention are useful for, e.g., research and diagnostic purposes.

REFERENCES:
patent: 4243507 (1981-01-01), Martin et al.
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4900414 (1990-02-01), Sibalis
patent: 4971670 (1990-11-01), Faupel et al.
patent: 5082548 (1992-01-01), Faupel et al.
patent: 5336387 (1994-08-01), Egen et al.
patent: 5376249 (1994-12-01), Afeyan et al.
patent: 5630924 (1997-05-01), Fuchs et al.
patent: 5773645 (1998-06-01), Hochstrasser
patent: 5916428 (1999-06-01), Kane et al.
patent: RE36350 (1999-10-01), Swedberg et al.
patent: 6013165 (2000-01-01), Wiktorowicz et al.
patent: 6017696 (2000-01-01), Heller
patent: 6103533 (2000-08-01), Hassard et al.
patent: 6123821 (2000-09-01), Anderson et al.
patent: 6136173 (2000-10-01), Anderson et al.
patent: 6240790 (2001-06-01), Swedberg et al.
patent: 6254754 (2001-07-01), Ross et al.
patent: 6277259 (2001-08-01), Guttman et al.
patent: 6329209 (2001-12-01), Wagner et al.
patent: 6638408 (2003-10-01), Speicher et al.
patent: 0 323 948 (1989-07-01), None
patent: 1 044 716 (1997-08-01), None
patent: WO 91/15773 (1991-10-01), None
patent: WO 91/17815 (1991-11-01), None
patent: WO 97/30346 (1997-08-01), None
patent: WO 00/04390 (2000-01-01), None
patent: WO 00/17631 (2000-03-01), None
patent: WO 00/31526 (2000-06-01), None
Bjellqvist et al. (“Isoelectric fousing in immobilized pH gradients: Principle, methodolgy and some applications,” Journal of Biochemical and Biophysical Methods, 6(1982) 317-339).
Righetti, et al., “Preparative protein purification in a multi-compartment electrolyser with immobiline membranes,”J. Chromatography475: 293-309 (1989).
Becker, H., et al., “Planar Quartz Chips With Submicron Channels for Two-Dimensional Capillary Electrophoresis Applications,” J. Micromech. Microeng. 8:24-28 (1998).
Daniels, M. and Landers, T. “Preparative-scale isoelectric purification of proteins without carrier ampholytes,” Science Tools from Pharmacia Biotech 1(2):3-5 (1996).
Gygi, S.O. et al., “Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology,” Proc. Natl. Acad. Sci. USA 97(17):9390-9395 (2000).
Islam, R., et al., “A new approach to rapid immobilized pH gradient IEF for 2-D electrophoresis.” Science Tools from Amersham Pharmacia Biotech 3(1):14-15 (1998).
Perrett, D., “Capillary Electrophoresis in Clinical Chamistry,” Ann. Clin. Biochem. 36:133-150 (1999).
Righetti, P.G., “Isoelectric Focusing: Theory, Methodology and Applications,” Laboratory Techniques in Biochemistry and Molecular Biology, Chapters 1 and 2; pp. 78-86, pp. 124-127 (1983).
Righetti, P.G., et al., “Protein Purification in Multicompartment Electrolyzers With Isoelectric Membranes,” J. Chromatography B 699:105-115 (1997).
Zuo, X., et al., (Aug. 2000) Quantitative evaluation of protein recoveries in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21(14):3035-47.
Zuo, X., et al., (Sep. 2000) A method for global analysis of complex proteomes using sample prefractionation by solution isoelectrofocusing prior to two-dimensional electrophoresis. Anal. Biochem. 284:266-278.
Zuo, X., et al., (May 2001) Towards global analysis of mammalian proteomes using sample prefractionation prior to narrow pH range two-dimensional gels and using one-dimensional gels for insoluble and large proteins. Electrophoresis 22(9):1603-15.
Zuo, X. et al., (Jan. 2002) Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two-dimensional electrophoresis. Proteomics 2(1):58-68.

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