Characterizing fluorescent and/or phosphorescent materials

Details Characterizing fluorescent and/or phosphorescent materials Characterizing fluorescent and/or phosphorescent materials

2007-09-25

2007-09-25

Porta, David (Department: 2884)

Radiant energy

Luminophor irradiation

Methods

C250S458100, C250S551000, C356S484000

Reexamination Certificate

active

11182050

ABSTRACT:
A method spectrally and temporally characterizing the fluorescence and/or phosphorescence of a material includes using a frequency-domain cross-correlation fluorometer-phosphorimeter employing an interferometer. A flux-modulated excitation signal is used to excite the material. An interferometer is used to generate an optical path difference (OPD) between portions of an emission signal produced by the excited material to obtain an optical interference signal. The optical interference signal is detected by a heterodyning detection system to determine phase shift and demodulation of the emission signal relative to the excitation signal. Phase shift and demodulation data are acquired at a series of OPD values generated by the interferometer and at a plurality of light source flux-modulation frequencies at each OPD to spectrally and temporally resolve the emission signal.

REFERENCES:
patent: 4840485 (1989-06-01), Gratton
patent: 5212386 (1993-05-01), Gratton et al.
patent: 5257202 (1993-10-01), Gratton et al.
patent: 5293213 (1994-03-01), Klein et al.
patent: 5323010 (1994-06-01), Gratton et al.
patent: 5416582 (1995-05-01), Knutson et al.
patent: 5485530 (1996-01-01), Lakowicz et al.
Gratton, E. et al, A Continuously Variable Frequency Cross-Correlation Phase Fluorometer with Picosecond Resolution, Biophys. J. 44, 315-324 (1983).
Feddersen, B. et al, Digital Parallel Acquisition in Frequency Domain Fluorometry, Rev. Sci. Inst. 60(9), 2929-2936 (1989).
Piston, D. et al, Wide-Band Acousto-Optic Light Modulator For Frequency Domain Fluorometry and Phosphorimetry, Rev. Sci. Inst. 60(8), 2596-2600 (1989).
Feddersen B Digital Frequency Domain Fluorometry and the Study of Hoechst 33258 Dye-DNA Interactions. PhD. Thesis, Dept. of Physics, University of Illinois at Urbana-Champaign (1993).
Fishkin, J. et al, Gigahertz Photon Density Waves in a Turbid Medium: Theory and Experiments, Phys. Rev. E. 53, 2307-2319 (1996).

Affiliated with

Also associated with

Rating

Characterizing fluorescent and/or phosphorescent materials does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Characterizing fluorescent and/or phosphorescent materials, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Characterizing fluorescent and/or phosphorescent materials will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3781241