Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2007-04-10
2007-04-10
Lankford, Jr., Leon B. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S123000, C435S105000, C435S253300
Reexamination Certificate
active
11195956
ABSTRACT:
A process for producing rhamnolipids involving culturingPseudomonas chlororaphisstrain NRRL B-30761 in a first aqueous culture medium containing about 0.3% NH4H2PO4, about 0.2% K2HPO4, about 0.2% carbon source, about 0.5 mg/L FeSO4, and about 0.1% MgSO4for about 24–about 48 hours at about 25°–about 30° C. with orbital shaking, and then culturingPseudomonas chlororaphisstrain NRRL B-30761 in a static second aqueous culture medium containing per liter about 2% carbon source, about 0.7 g KH2PO4, about 0.9 g Na2HPO4, about 2 g NaNO3, about 0.4 g MgSO4.7H2O, and about 0.1 g CaCl2.2H2O for at least about 72 hours at about 20°–about 23° C., wherein the first and second aqueous culture medium contains only one source of carbon.
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Fett William F.
Gunther Nereus W.
Solaiman Daniel K. Y.
Fado John D.
Kim Taeyoon
Lankford , Jr. Leon B.
Poulos Gail E.
United States of America as represented by the Secretary of Agri
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