Method of assaying lupus anticoagulant and assay reagent

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S007920, C530S387100, C530S387700, C530S388200

Reexamination Certificate

active

10472283

ABSTRACT:
A monoclonal antibody having inhibitory activity on blood coagulation reactions, which reacts with prothrombin forming a complex with phosphatidylserine but does not react with prothrombin not forming a complex with phosphatidylserine; and a method for measuring inhibitory activity of test plasma on blood coagulation reactions, which comprises the steps of (1) measuring inhibitory activity of the test plasma on the blood coagulation reactions and inhibitory activity of this on the blood coagulation reactions, and (2) comparing the inhibitory activity of the test plasma with the inhibitory activity of this antibody.

REFERENCES:
patent: WO 00/02925 (2000-01-01), None
Galil et al. Thrombosis and Haemostasis 1999 vol. 77, p. 486-91.
Atsumi, et al., “Association of Autoantibodies Against the Phosphatidylserine-Prothrombin Complex with Manifestations of the Antiphospholipid Syndrome and with the Presence of Lupus Anticoagulant”,Arthritis&Rheumatism, 43(9): 1982-1993, 2000.
Atsumi, et al., “Anti Prothrombin-Phosphatidylserine Antibodies and Their Association with Lupus Anticoagulant in Japanese Patients with Autoimmune Diseases”, Abstract. No. B077.
Atsumi, et al., “Characteristics of Phosphatidylserine-Dependent Mouse Monoclonal Antiprothrombin Antibodies”, Abstract. No. P32.
Atsumi, et al., “Clinical Significance of Anti Prothrombin-Phosphatidylserine Antibodies and Their Association with Lupus Anticoagulant in Patients with Autoimmune Diseases”, Abstract. No. 821.
Journal of Autoimmunity, vol. 15, No. 2, Sep. 2000, p. A38, XP009033955 9thInternational Symposium on Antiphospholipid Antibodies; Tours, France; Sep. 12-16, 2000 ISSN 0896-8411.
“A Monoclonal Antibody Against Prothrombin Fragment 1 Behaves Like a Lupus Anticoagulant,” Thombosis and Haemostasis, Stuttgart, DE, 81(3): Mar. 1999, 470-471.
Matsuda et al., “Phosphatidyl Serine-Dependent Antiprothrombin Antibody is Exclusive to Patients with Lupus Anticoagulant,”British Journal of Rheumatology,35(6): 589-591, 1996.
International Search Report for EP 02 70 5339.

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