Optics: measuring and testing – Plural test
Reexamination Certificate
2007-01-09
2007-01-09
Stafira, Michael P. (Department: 2877)
Optics: measuring and testing
Plural test
C356S338000
Reexamination Certificate
active
10849477
ABSTRACT:
An imaging fountain flow cytometer allows high resolution microscopic imaging of a flowing sample in real time. Cells of interest are in a vertical stream of liquid flowing toward one or more illuminating elements at wavelengths which illuminate fluorescent dyes and cause the cells to fluoresce. A detector detects the fluorescence emission each time a marked cell passes through the focal plane of the detector. A bi-directional syringe pump allows the user to reverse the flow and locate the detected cell in the field of view. The flow cell is mounted on a computer controlled x-y stage, so the user can center a portion of the image on which to zoom or increase magnification. Several computer selectable parfocal objective lenses allow the user to image the entire field of view and then zoom in on the detected cell at substantially higher resolution. The magnified cell is then imaged at the various wavelengths.
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Bales Jennifer L.
Macheledt Bales & Heidmiller LLP
Stafira Michael P.
University of Wyoming
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