Engineering of metabolic control

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252330, C435S006120, C435S071100, C435S233000, C536S023200, C536S024100

Reexamination Certificate

active

07122341

ABSTRACT:
The invention features a method of producing heterologous molecules in cells under the regulatory control of a metabolite and metabolic flux. The method can enhance the synthesis of heterologous polypeptides and metabolites.

REFERENCES:
patent: 5429939 (1995-07-01), Misawa et al.
patent: 5530189 (1996-06-01), Ausich et al.
patent: 5744341 (1998-04-01), Cunningham, Jr. et al.
patent: 5830692 (1998-11-01), Bock et al.
patent: 5906925 (1999-05-01), Liao
patent: 2002/0045220 (2002-04-01), Khosla et al.
patent: 96/08567 (1996-03-01), None
S. Kajiwara et al. “Expression of an Exogenous Isopentenyl Diphosphate Isomerase Gene Enhances Isoprenoid Biosynthesis iEscherichia coli”, Biochemical J. 324: 421-426. (1997).
Campos-Garcia et al., “Thepseudomonas aeruginosa rhlGgene encodes an NADPH-dependent β-Ketoacyl which is specifically involved in rhamnolipid synthesis”,Journal of Bacteriology180 (17):4442-4451 (1998).
Farmer et al., “Reprogramming the regulatory circuits ofEscherichia coli”, Abstract 083., American Chemical Society National Meeting, Boston, MA Aug. 23-27 (1998).
Farmer et al., “Reprogramming the regulatory circuits ofEscherichia coli”, Abstract 094., American Chemical Society National Meeting, Anaheim, CA Mar. 21-25 (1999).
Feng et al., “Role of phosphorylated metabolic intermediates in the regulation of glutamine synthetase synthesis inEscherichia coli”, Journal of Bacteriology174(19):6061-6070 (1992).
Haldimann et al., “Transcriptional regulation of theEnterococcus faeciumBM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system inEscherichia coliK-12”,Journal of Bacteriology179(18):5903-5913 (1997).
McCleary et al., “Acetyl phosphate a global signal inEscherichia coli?”, Journal of Bacteriology175(10):2793-2798 (1993).
McCleary et al., “Acetyl phophate and the activation of two-component response regulators”,Journal of Biological Chemistry269(50):31567-31572 (1994).
Misawa et al., “Elucidation of theErwinia uredovoracarotenoid biosynthetic pathway by functional analysis of gene products expressed inEscherichia coli”, Journal of Bacteriology172(12):6704-6712 (1990).
Ruther et al., “Production of zeaxanthin inEscherichia colitransformed with different carotenogenic plasmids”,Appl Microbiol Biotechnol48:162-167 (1997).
Schroeckh et al., “Formation of recombinant proteins inEscherichia coliunder control of a nitrogen regulated promoter at low and high cell densities”,Journal of Biotechnology49:45-58 (1996).
Scroeckh et al., “The use of elements of theE. coliNtr-system for the design of an optimized recombinant expression system for high cell density cultivations”.Journal of Biotechnology75:241-250 (1999).
Shin et al., “Modulation of flagellar expression inEscherichia coliby acetyl phosphate and the osmoregulator OmpR”,Journal of Bacteriology177(16):4696-4702 (1995).
Sprenger et al., “Identification of a thiamin-dependent synthase inEscherichia colirequired for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol”,Proc. Natl. Acad. Sci USA94:12857-12862 (1997).
Wang et al., “Engineered Isoprenoid pathway enhances Astaxanthin production inEscherichia coli”, Biotechnology and Bioengineering62(2):235-241 (1999).
Alex and Simon, “Protein histidine kinases and signal transduction in prokaryotes and eukaryotes”,Trends in Genetics10(4):133-138 (1994).
Aristidou et al., “Metabolic engineering ofEscherichia colito enhance recombinant protein production through acetate reduction”,Biotechnol. Prog.11:475-478 (1995).
Bauer et al., “Improved expression of human interleukin-2 in high-cell-density fermentor cultures ofEscherichia coliK-12 by a phosphotransacetylase mutant”,Appl Environ Microbiol.56(5):1296-1302 (1990).
DeWitt et al., This Month inNature Biotechnology, Nature Biotechnology18:480 (2000).
Diez-Gonzalez and Russell, “The ability ofEscherichia coliO157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid”,Microbiology143:1175-1180 (1997).
Farmer and Liao, “Improving lycopene production inEscherichia coli byengineering metabolic control,”Nature Biotechnol.18:533-537 (2000).
Hakenbeck and Stock, “Analysis of Two-Component Signal Transduction Systems Involved in Transcriptional Regulation”,Methods in Enzymology273:281-301 (1996).
Parkinson and Kofoid, “Communication Modules in Baterial Signaling Proteins”,Annu. Rev. Genet.26:71-112 (1992).
Sevenich et al., “DNA binding and oligomerization of NtrC studied by fluorescence anisotropy and fluorescence correlation spectroscopy”,Nucleic Acid Res.26(6):1373-1381 (1998).
Reitzer et al., “Expression ofglnAinEscherichia coliis regulated at tandem promoters”,Proc. Natl. Acad. Sci. USA82:1979-1983 (1985).
Wanner et al., “Involvement of Phosphotransacetylase, Acetate Kinase, and Acetyl Phosphate Synthesis in Control of the Phosphate Regulon inEscherichia coli”, J. Bacteriol.174(7):2124-2130 (1992).
Albrecht, M. et al., “Metabolic Engineering of the Terpenoid Biosynthetic Pathway ofEcherichia colifor Production of the Carotenoids β-Carotene and Zeaxanthin”,Biotechnology Lettersvol. 21, pp. 791-795, XP009007288; 1999.
Matthews, P.D and Wurtzel E.T., “Metabolic Engineering of Carotenoid Accumulation inEcherichia coliby Modulation of the Isoprenoid Precursor pool with Expression of Deoxyxylulose Phosphate Synthase”,Applied Microbiology Biotechnology,vol. 53, pp. 396-400, XP-000941210; 2000.
Misawa N. and Shimada H., “Metabolic Engineering for the Production of Carotenoids in Non-Carotenogenic Bacteria and Yeasts”,Journal of Biotechnology, vol. 59, pp. 169-181, 1998.
Patnaik R. and Liao, J.C., “Engineering ofEcherichia coliCentral Metabolism for Aromatic Metabolite Production with Near Theoretical Yield”,Applied and Environmental Microbiology,vol. 60, No. 11, pp. 3903-3908, XP 000610868, 1994.
Patnaik, R. et al., “Pathway Engineering for Production of Aromatics inEcherichia coli: Confirmation of Stoichiometric Analysis by Independent Modulation of AroG, TktA, and Pps Activities”,Biotechnology and Bioingineering,vol. 46, pp. 361-370, XP-000882934, 1995.
Sandmann, G. et al., “The Biotechnological Potential and Design of Novel Carotenoids by Gene Combination inEcherichia coli”, Trends in Biotechnology, Reviews,vol. 17, pp. 233-237, 1999.
Schmidt-Dannert, C., “Engineering Novel Carotenoids in Microorganisms”,Current Opinion in Biotechnology,vol. 11, pp. 255-261, XP000985860, 2000.
Shimada, H. et al., “Increased Carotenoid Production by the Food YeastCandida utilisthrough Metabolic Engineering of Isoprenoid Pathway”,Applied and Environmental Microbiology,vol. 64, No. 7, pp. 2676-2680, XP-002269481, 1998.
Communication dated Feb. 26, 2004 for EP 00 95 0804.5 (8 pages).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Engineering of metabolic control does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Engineering of metabolic control, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Engineering of metabolic control will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3704295

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.