Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Reexamination Certificate
2006-11-07
2006-11-07
Chen, Stacy B. (Department: 1648)
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
C435S239000, C424S204100
Reexamination Certificate
active
07132271
ABSTRACT:
Methods for enhancing the production of viral vaccines in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain interferon induced antiviral activities, in particular, cellular levels of double-stranded RNA (dsRNA) dependent kinase (PKR) and 2′-5′ oligoadenylate synthetase (2-5A synthetase). In cell cultures deficient for PKR or 2-5A synthetase, viral yield is enhanced by several orders of magnitude over cell cultures with normal levels of these proteins making these cell cultures useful for the production of viral vaccines.
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Samuel, Charles E.; Ozato, Keiko, “induction of interferons and interferon-induced genes,&#
Bozicevic Field & Francis LLP
Chen Stacy B.
Francis Carol L.
The Regents of the University of California
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