Reference control containing a nucleated red blood cell...

Details Reference control containing a nucleated red blood cell... Reference control containing a nucleated red blood cell...

2006-11-14

2006-11-14

Wallenhorst, Maureen M. (Department: 1743)

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

C436S008000, C436S063000, C436S174000, C436S175000, C436S017000, C436S018000, C435S002000

Reexamination Certificate

active

07135341

ABSTRACT:
A method of making a reference control containing a nucleated red blood cell component includes providing a blood cell containing a nucleus; treating the blood cell with a treatment solution to alter a nucleus property from a natural value to a target value suitable for simulating nucleated red blood cells on a blood analyzer; and suspending treated blood cell in a suspension medium to form a reference control. The method also includes integrating the nucleated red blood cell component with white blood cell, red blood cell, platelet and reticulocyte components. Further disclosed is a cell treatment composition for altering a nucleus property, which includes a conditioning component, a lytic component for permeating cell membrane, and a fixing component for preserving the cell nucleus. Also disclosed is a method of using the reference control for measurement of nucleated red blood cells on a blood analyzer.

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