Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism
Patent
1985-09-09
1989-09-19
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Treating animal or plant material or micro-organism
435 1, 424110, C07G 1500, A01N 102
Patent
active
048681210
ABSTRACT:
A method of producing intact islets of Langerhans in an insulin producing condition uses a mixture of Hank's solution and 10% by volume fetal calf serum to ductally distend the human pancreas. The exocrine tissue of the pancreas is digested at about 37.degree. C. by an enzyme preparation of collagenase, trypsin and proteolytic enzyme present in the mixture at a level of about 0.2% by weight. The digestion is monitored at regular intervals during the process. The digested pancreas is comminuted, filtered and intact islets of Langerhans are recovered. The recovered islets retain their insulin producing properties.
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"New Approaches in the Methods for Isolating Mammalian Islets of Langerhans Utilizing the Dog Pancreas," Chem Abstracts, 95:130315d, 1981, Scharp, et al.
"Method for Isolation of Islets of Langerhans from the Human Pancreas," Diabetes 33:1055, 1984, Gray et al.
Finke Edward H.
Lacy Paul E.
Poteat Thomas J.
Scharp David W.
Kepplinger Esther M.
McDonnell Douglas Corporation
Washington University
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