Liquid purification or separation – Processes – Separating
Patent
1988-07-12
1989-09-19
Jones, W. Gary
Liquid purification or separation
Processes
Separating
210516, 210789, 422101, 436177, 494 37, B01D 2126
Patent
active
048678872
ABSTRACT:
A blood separation device includes a collection tube containing a first layer of a thixotropic gel-like substance and a second layer of a newtonian gel-like substance which is positioned below the first layer. The first layer of the thixotropic substance is positioned between the second layer of the newtonian substance and a blood sample received by the tube, prior to centrifuging the tube, and acts as a barrier between the second layer and the blood sample to prevent intermixing of the second layer and the blood sample. The thixotropic gel-like substance of the first layer has a specific gravity which is greater than the newtonian gel-like substance of the second layer whereupon centrifugation of the tube, the first layer moves to a position below the second layer. The second layer of the newtonian gel-like substance acts as a density separation medium, the newtonian gel-like substance being formed with a specific gravity such that the second layer is adapted to assume a position in the collection tube, upon centrifugation of the tube, between the mononuclear cells and the heavier components of the blood sample placed in the tube.
REFERENCES:
patent: 3852194 (1974-12-01), Zine, Jr.
patent: 3920549 (1975-11-01), Gigliello et al.
patent: 3945928 (1976-03-01), Ayres
patent: 3960727 (1976-06-01), Hochstrasser
patent: 4101422 (1978-07-01), Lamont et al.
patent: 4147628 (1979-04-01), Bennett et al.
patent: 4153739 (1979-05-01), Kessler
patent: 4190535 (1980-02-01), Luderer et al.
patent: 4255256 (1981-03-01), Ferrante et al.
patent: 4310430 (1982-01-01), Ichikawa et al.
patent: 4350593 (1982-09-01), Kessler
patent: 4417981 (1983-11-01), Nugent
patent: 4435293 (1984-03-01), Graham, Jr. et al.
patent: 4436631 (1984-03-01), Graham, Jr. et al.
patent: 4457782 (1984-07-01), Honda et al.
patent: 4487700 (1984-12-01), Kanter
patent: 4534798 (1985-08-01), Honda et al.
patent: 4640785 (1987-02-01), Carroll et al.
patent: 4751001 (1988-06-01), Saunders
patent: 4816168 (1989-03-01), Carrol et al.
patent: 4818418 (1989-04-01), Sauders
Iodinated Density Gradient Media, by Dr. D. Rickwood, Chapter 7, by IRL Press, (1983).
Rapid, Quantitative Human Lymphocyte Separation and Purification in a Closed System, by Luderer et al., Molecular Immunology, 16, pp. 621-624, (1979).
Comparison of T and B Cell Analyses on Fresh and Aged Blood, by J. K. A. Nicholson et al., Journal of Immunological Methods, 73, pp. 29-40, (1984).
Experimental Cell Research, by Splinter et al., (1978), pp. 245-251.
9265X, Chemical Abstract.
Becton Dickinson and Company
Jones W. Gary
LandOfFree
Method and apparatus for separating mononuclear cells from blood does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and apparatus for separating mononuclear cells from blood, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and apparatus for separating mononuclear cells from blood will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-367089