Method of analyzing a target nucleic acid

Details Method of analyzing a target nucleic acid Method of analyzing a target nucleic acid

2006-10-03

2006-10-03

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S025320

Reexamination Certificate

active

07115365

ABSTRACT:
A biological sample containing a target nucleic acid is mixed with a primer, a substrate labeled with fluorescence, and DNA polymerase to prepare a test solution. After a nucleic acid amplification reaction is performed in a predetermined manner, the test solution is attached dropwise on a slide glass, which is mounted on a sample holder of an inverted fluorescence microscope. Data measured by a photomultiplyer are arithmetically operated by applying an autocorrelation function in a data processing apparatus. Based on the arithmetic results, quantification data for the target nucleic acid, are numerically or graphically displayed on a screen of a display apparatus.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5567583 (1996-10-01), Wang et al.
patent: 5807677 (1998-09-01), Eigen et al.
patent: 6391544 (2002-05-01), Salituro et al.
Gyllensten et al., generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus, Proc. Natl. Acad. Sci. USA, 1988, vol. 85, p. 7652-7656.
Gyllensten et al., “Generation of single-stranded DNA by the polymerase chain reaction and its application . . . sequencing of the HLA-DQA locus”, Pro. Natl. Acad. Sci., vol. 85, pp. 7652-7656 (1988).
Clive et al., J. Neurosci. Methods, 1998, vol. 81, (1,2), p. 25-34.
McCabe, PCR Protocol: A Guide to Methods and Applications, Part one, 1990, Chapter 10, p. 76-83.

Affiliated with

Also associated with

Rating

Method of analyzing a target nucleic acid does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of analyzing a target nucleic acid, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of analyzing a target nucleic acid will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3662508