Method for classifying object image regions of an object to...

Radiant energy – Luminophor irradiation

Reexamination Certificate

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C372S006000, C372S025000

Reexamination Certificate

active

07151270

ABSTRACT:
A method for classifying a plurality of object image regions of an object to be detected using a scanning microscope includes labeling each of the image areas of the object with a different marker so that light of a respective characteristic emanates from each marker. The intensity of the light from each marker is detected using at least two channels so as to generate detection signals, each detection signal being a function of the intensity of the detected light. The object regions are classified using ratios of the detection signals.

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“Bio-Imaging: Multi-Spectral Imaging and Linear Unmixing Add a Whole New Dimension to Laser Scanning Fluorescence Microscopy”, M.E. Dickinson et al. BioTechniques 2001, vol. 31, No. 6, 1272-1278.

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